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Anti-inflammatory Effect of Shea Butter Extracts in Canine Keratinocytes

한국임상수의학회지 2021년 38권 1호 p.27 ~ 31
임다혜, 배슬기, 오태호,
소속 상세정보
임다혜 ( Lim Da-Hye ) - Kyungpook National University College of Veterinary Medicine
배슬기 ( Bae Seul-Gi ) - Kyungpook National University College of Veterinary Medicine
오태호 ( Oh Tae-Ho ) - Kyungpook National University College of Veterinary Medicine

Abstract


Shea butter (Vitellaria paradoxa) is a fat extracted from shea tree nuts and contains relatively high levels of non-glycerides. Triterpenes, the main non-glyceride component, exhibit a variety of biological activities such as antitumor, antibacterial, and anti-inflammatory. Shea butter extract (SBE) has been used to treat various skin problems such as burns, eczema, and rash in human medicine, but little is known about the activity of SBE on canine skin. This study evaluated the cytotoxicity and anti-inflammatory effect of SBE in canine keratinocytes. Cytotoxicity of lipopolysaccharide (LPS, 5-50 ng/mL) and SBE (50-200 ?g/mL) was evaluated using the CCK-8 assay. Non-cytotoxic concentrations of LPS and SBE were administered to canine cell cultures to evaluate anti-inflammatory effects. To evaluate the anti-inflammatory activity of SBE, the levels of IL-1?, IL-8, IL-12, and TNF-? were measured using ELISA kits. The concentration of each cytokine was quantified in control, LPS-treated, LPS + SBE-treated groups. Increased levels of IL-1?, IL-8, and IL-12 were found in LPS-treated groups relative to control groups. LPS + SBEtreated groups showed a lower level of IL-1?, IL-8, and IL-12 than LPS-treated groups. These results suggest that SBE may have application as a topical agent for canine inflammatory skin diseases. However, further in vivo study is needed to evaluate the safety and efficacy of SBE in dogs.

키워드

Shea butter extracts (SBE); anti-inflammatory; cytokine; keratinocyte; dog

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