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Aptamer-immobilized Gold Nanoparticles Enable Facile and On-site Detection of Staphylococcus aureus

Biotechnology and Bioprocess Engineering 2021년 26권 1호 p.107 ~ 113
임성하, 류영채, 황병희,
소속 상세정보
임성하 ( Lim Seong-Ha ) - Incheon National University Department of Chemistry
류영채 ( Ryu Yeong-Chae ) - Incheon National University Department of Bioengineering and Nano-Bioengineering
황병희 ( Hwang Byeong-Hee ) - Incheon National University Department of Bioengineering and Nano-Bioengineering

Abstract


Staphylococcus aureus causes numerous illnesses and national loss in many countries. Therefore, to prevent food poisoning outbreaks, a rapid and straightforward on-site detection method is required without special skills or equipment. Here, we used aptamer-immobilized gold nanoparticles (aiGNPs) for the on-site colorimetric S. aureus detection. For the rapid and facile detection of S. aureus, the SA61 aptamer hybridized with thiol-modified adaptors conjugated onto the GNP surface of approximately 13 nm diameter. The synthesis of GNPs was confirmed using a transmission electron microscopy and energy dispersive spectroscopy. Images using a scanning electron microscope showed attachment of aiGNPs to S. aureus. After 30-min incubation and 20-min centrifugation, the red pellet was specifically observed with the sensitivity of OD600 0.050 equivalent to 7.5 × 106 cells/mL. Besides, aiGNPs could accurately detect S. aureus with a detection limit of OD600 0.10 equivalent to 1.5 × 107 cells/mL even in the mixed sample with Escherichia coli. Quantitative analysis was performed between relative area and cell concentration. Because this method enables fast and specific S. aureus detection without special skills or expensive equipment, aiGNPs could enable a rapid and effective on-site detection of S. aureus to prevent pathogenic outbreaks.

키워드

aptamer; gold nanoparticle; on-site detection; Staphylococcus aureus; diagnosis

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