잠시만 기다려 주세요. 로딩중입니다.

Evaluation of the CellaVision Advanced RBC Application for Detecting Red Blood Cell Morphological Abnormalities

Annals of Laboratory Medicine 2021년 41권 1호 p.44 ~ 50
박성준, Yoon Jung, 권정아, 윤수영,
소속 상세정보
박성준 ( Park Seong-Jun ) - Korea University Guro Hospital Department of Laboratory Medicine
 ( Yoon Jung ) - Korea University Guro Hospital Department of Laboratory Medicine
권정아 ( Kwon Jung-Ah ) - Korea University Guro Hospital Department of Laboratory Medicine
윤수영 ( Yoon Soo-Young ) - Korea University Guro Hospital Department of Laboratory Medicine

Abstract


Background: The Advanced RBC Application of the CellaVision DM9600 system (CellaVision AB, Lund, Sweden) automatically characterizes and classifies red blood cells (RBCs) into 21 morphological categories based on their size, color, shape, and inclusions. We evaluated the diagnostic performance of the CellaVision Advanced RBC Application with respect to the classification and grading of RBC morphological abnormalities in accordance with the 2015 International Council for Standardization in Haematology (ICSH) guidelines.

Methods: A total of 223 samples, including 123 with RBC morphological abnormalities and 100 from healthy controls, were included. Seven RBC morphological abnormalities and their grading obtained with CellaVision DM9600 pre- and post-classification were compared with the results obtained using manual microscopic examination. The grading cut-off percentages were determined in accordance with the 2015 ICSH guidelines. The sensitivity and specificity of the CellaVision DM9600 system were evaluated using the manual microscopic examination results as a true positive.

Results: In pre-classification, >90% sensitivity was observed for target cells, tear drop cells, and schistocytes, while >90% specificity was observed for acanthocytes, spherocytes, target cells, and tear drop cells. In post-classification, the detection sensitivity and specificity of most RBC morphological abnormalities increased, except for schistocytes (sensitivity) and acanthocytes (specificity). The grade agreement rates ranged from 35.9% (echinocytes) to 89.7% (spherocytes) in pre-classification and from 46.2% (echinocytes) to 90.1% (spherocytes) in post-classification. The agreement rate of samples with within-one grade difference exceeded 90% in most categories, except for schistocytes and echinocytes.

Conclusions: The Advanced RBC Application of CellaVision DM9600 is a valuable screening tool for detecting RBC morphological abnormalities.

키워드

Red blood cell; Morphology; Advanced RBC Application; CellaVision DM9600; International Council for Standardization in Haematology guidelines

원문 및 링크아웃 정보

등재저널 정보