잠시만 기다려 주세요. 로딩중입니다.

오령산 정제 개발 및 항염증 효과

Effect of Anti-inflammation on Oryeong-san Formulation for Mix Extract Tablet

동의생리병리학회지 2020년 34권 6호 p.348 ~ 354
김세진, 임현희, 남원희, 손수미, 최혜민, 김명진, 김정옥, 이화동,
소속 상세정보
김세진 ( Kim Se-Jin ) - National Institute for Korean Medicine Development
임현희 ( Leem Hyun-Hee ) - National Institute for Korean Medicine Development
남원희 ( Nam Won-Hee ) - National Institute for Korean Medicine Development
손수미 ( Son Su-Mi ) - National Institute for Korean Medicine Development
최혜민 ( Choi Hye-Min ) - National Institute for Korean Medicine Development
김명진 ( Kim Myung-Jin ) - National Institute for Korean Medicine Development
김정옥 ( Kim Jung-Ok ) - National Institute for Korean Medicine Development
이화동 ( Lee Hwa-Dong ) - National Institute for Korean Medicine Development

Abstract


Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.

키워드

Oryeong-san (ORS)(五?散); Tablet; Simultaneous analysis; Anti-inflammation; Cytokines

원문 및 링크아웃 정보

 

등재저널 정보