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한방약침소재로써 속수자 초임계추출물의 항산화 및 항노화에 대한 연구

Anti-Oxidant and Anti-Aging Effect of Supercritical Fluid Extraction of Seed of Euphorbia lathyris L. as a Pharmacopuncture Material

경락경혈학회지 2020년 37권 2호 p.88 ~ 96
곽병문, 김태준, 김이화,
소속 상세정보
곽병문 ( Kwak Byeong-Mun ) - Semyung University College of Korean Medicine Deptartment of Meridian and Acupoint
김태준 ( Kim Tae-Jun ) - Semyung University College of Korean Medicine Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology
김이화 ( Kim Ee-Hwa ) - Semyung University College of Korean Medicine Deptartment of Meridian and Acupoint

Abstract


Objectives: : The purpose of this study was to investigate the anti-oxidant and anti-aging effect of the seed of Euphorbia lathyris L. extracted by supercritical CO2.

Methods : Human dermal fibroblast cells dosed with the extract from Euphorbia lathyris L. were harvested and the intracellular proteome was analyzed to examine the expression of proteins related collagen synthesis pathway, metalloproteinases (MMPs), extracellular matrix (ECM)-cell interaction, cytokines, and antioxidant enzymes by 2-dimensional gel electrophoresis.

Results : Fatty acid analysis of the extract from Euphorbia lathyris L. showed oleic acid was 84% and linoleic acid was 4.1%. Antioxidative effect was about 53% by beta carotene bleaching assay. In 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis, fifteen protein changes in five mechanisms which were collagen synthesis pathway, MMPs, ECM-cell interaction, cytokines, and antioxidant enzymes were analyzed.

Conclusions : This study suggests the supercritical extraction from the seed of Euphorbia lathyris L. could be used as anti-oxidant substances for pharmacopuncture.

키워드

Euphorbia lathyris L.; supercritical fluid extraction; anti-oxidant

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