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Wnt/β-catenin 신호 활성화를 통한 미세전류 자극의 지방생성 억제 효과

The Micro-Current Stimulation Inhibits Adipogenesis by Activating Wnt/β-Catenin Signaling

Journal of Biomedical Engineering Research 2020년 41권 6호 p.235 ~ 246
황동현, 이한아, 이민주, 조승관, 김한성,
소속 상세정보
황동현 ( Hwang Dong-Hyun ) - Yonsei University Department of Biomedical Engineering
이한아 ( Lee Han-A ) - Yonsei University Department of Biomedical Engineering
이민주 ( Lee Min-Joo ) - Yonsei University Department of Biomedical Engineering
조승관 ( Cho Seung-Kwan ) - CELLOGIN Inc.
김한성 ( Kim Han-Sung ) - Yonsei University Department of Biomedical Engineering

Abstract


This study aimed to evaluate the inhibitory effect of micro-current stimulation(MCS) on adipogenesis regarding with Wnt/β-catenin pathway using the ob/ob mouse and 3T3-L1 cell line. 6-week old ob/ob male mice were equally assigned to four groups: obese group(ob), obese with MCS groups(50 μA, 200 μA, and 400 μA). 6-week old C57BL/6J male mice were assigned to the control group(CON). We analyzed abdominal adipose tissue volume by using in vivo micro-CT and measured the body weight, feed intake, liver weight and triglycerides in serum. All the MCS groups showed that significantly reduced body weight and triglycerides in serum. In the case of liver weight and abdominal adipose tissue volume, the inhibitory effect of adipogenesis was shown in the 200 μA and 400 μA groups. To elucidate the anti-obesity effect of MCS, β-catenin, C/EBPα and FAS protein expressions were analyzed by western blotting. β-catenin expression was upregulated, C/EBPα and FAS expression were down-regulated in the relatively high-intensity groups(200 μA and 400 μA). Thus, the 200 μA and 400 μA for the intensity of MCS were chosen for cell experiments. In the 3T3-L1 cell line, Wnt/β-catenin pathway including Wnt10b, Wnt3a, β-catenin and Cyclin D1 was activated in all MCS groups. Accordingly, the expression level of C/EBPα was decreased during the differentiation and lipid droplet was significantly reduced in Oil red O staining results. These results suggest that the Wnt/β-catenin signaling might be activated by MCS with current intensities between 200-400 μA and it may lead to anti-obesity effects.

키워드

Adipogenesis; Micro-current stimulation; Wnt/β-catenin pathway; ob/ob; 3T3-L1

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