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Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

Journal of Gastric Cancer 2020년 20권 3호 p.300 ~ 312
Shen Duo, Zhao Hongyu, Zeng Peng, Song Jinyun, Yang Yiqiong, Gu Xuefeng, Ji Qinghua, Zhao Wei,
소속 상세정보
 ( Shen Duo ) - Southeast University Medical School
 ( Zhao Hongyu ) - Nanjing University of Chinese Medicine Second Hospital of Nanjing Department of Clinical Research Center
 ( Zeng Peng ) - Southeast University Medical School
 ( Song Jinyun ) - Nanjing University of Chinese Medicine Second Hospital of Nanjing Department of Clinical Research Center
 ( Yang Yiqiong ) - Southeast University Medical School
 ( Gu Xuefeng ) - Southeast University Medical School
 ( Ji Qinghua ) - Southeast University Medical School
 ( Zhao Wei ) - Southeast University Medical School

Abstract


Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556.

Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer.

Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice.

Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

키워드

CircRNAs; MMP19; miRNA sponge; Gastric cancer

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