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Acinetobacter baumannii와 Candida albicans의 배양시간에 따른 자가형광발현과 pH의 변화 비교

Comparison of Autofluorescence and pH Changes in Acinetobacter baumannii and Candida albicans Cultures based on Incubation Time

한국임상치위생학회지 2021년 9권 1호 p.11 ~ 19
이윤선, 한선영,
소속 상세정보
이윤선 ( Lee Yun-Seon ) - Yonsei University Department of Dental Hygiene
한선영 ( Han Sun-Young ) - Yonsei University Department of Dental Hygiene

Abstract


Objectives: Quantitative Light-induced Fluorescence-Digital (QLF-D) was used to investigate the relationship between the autofluorescence characteristics of Acinetobacter baumannii and Candida albicans (a major causative agent and a risk factor for nosocomial pneumonia, respectively) and pH change.

Methods: A. baumannii and C. albicans were the experimental microorganisms, and Streptococcus mutans was the control. Autofluorescence expression was assessed by smearing the cells on a solid medium and imaging for QLF-D at 48 h intervals for 168 h. The R/G ratio was calculated for the three types of colonies. The pH of the cultures (in liquid media) was measured for each incubation time. For statistical analysis, the Kruskal-Wallis test and Pearson correlation analysis were performed using SPSS version 25.0.

Results: A. baumannii emitted orange fluorescence; the R/G ratio and pH increased at 24 h and peaked at 168 h, and exhibited positive correlation. C. albicans emitted reddish fluorescence; the R/G ratio and pH increased at 24 h, peaked at 120 h, and subsequently decreased. S. mutans did not exhibit fluorescence.

Conclusions: The fluorescence of the A. baumannii cells was clearly observed, and the pH of the medium increased. The pH of the C. albicans culture peaked at 120 h and then decreased gradually. The correlation between autofluorescence and pH was confirmed.

키워드

아시네토박터 바우마니, 캔디다 알비칸스, 형광, 수소이온농도, 흡인성폐렴, 정량광형광기
Acinetobacter baumannii; Candida albicans; Fluorescence; pH; Aspiration Pneumonia; Quantitative Light-Induced Fluorescence

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