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Isolation of mesenchymal stem cells from Pap smear samples

Obstetrics & Gynecology Science 2020년 63권 5호 p.594 ~ 604
박중현, 손다련, 홍원준, 장지훈, 조금준, 송권화, 김인용, 유승권,
소속 상세정보
박중현 ( Park Jung-Hyun ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
손다련 ( Son Da-Ryeon ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
홍원준 ( Hong Won-Jun ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
장지훈 ( Jang Ji-Hoon ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
조금준 ( Cho Geum-Joon ) - Korea University College of Medicine Department of Obstetrics and Gynecology
송권화 ( Song Gwon-Hwa ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
김인용 ( Kim In-Yong ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology
유승권 ( You Seung-Kwon ) - Korea University College of Life Sciences and Biotechnology Department of Biotechnology

Abstract


Objective: Exploiting their ability to differentiate into mesenchymal lineages like cartilage, bone, fat, and muscle, and to elicit paracrine effects, mesenchymal stem cells (MSCs) are widely used in clinical settings to treat tissue injuries and autoimmune disorders. One of accessible sources of MSC is the samples used for Papanicolaou (Pap) test, which is a cervical screening method for detecting potentially pre-cancerous and cancerous alterations in the cervical cells and to diagnose genetic abnormalities in fetuses. This study aimed to identify and isolate the stem cells from Pap smear samples collected from pregnant women, and to trace the origin of these cells to maternal or fetal tissue, and characterize their stem cell properties.

Methods: To investigate the possibility and efficiency of establishing MSC lines from the Pap smear samples, we were able to establish 6 cell lines from Pap smear samples from 60 pregnant women at different stages of gestation.

Results: The 3 cell lines randomly selected among the 6 established in this study, displayed high proliferation rates, several characteristics of MSCs, and the capacity to differentiate into adipocytes, osteocytes, and chondrocytes. Our study identified that the stem cell lines obtainable from Pap smear sampling were uterine cervical stromal cells (UCSCs) and had 10% efficiency of establishment.

Conclusion: Despite their low efficiency of establishment, human UCSCs from Pap smear samples can become a simple, safe, low-cost, and donor-specific source of MSCs for stem cell therapy and regenerative medicine.

키워드

Papanicolaou test; Mesenchymal stem cells; Regenerative medicine

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