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Camellia sinesis leaves extract ameliorates high fat diet-induced nonalcoholic steatohepatitis in rats: analysis of potential mechanisms

Journal of Pharmaceutical Investigation 2021년 51권 2호 p.183 ~ 197
Mohamed Safaa H., Shahat Abdelaaty A., Mohamed Mohamed R., Khalil Wagdy K. B., Salem Ahmed M., Farrag Abdel Razik H., Ahmed Hanaa H.,
소속 상세정보
 ( Mohamed Safaa H. ) - Hormones Department, National Research Center, Dokki
 ( Shahat Abdelaaty A. ) - National Research Centre Phytochemistry Department
 ( Mohamed Mohamed R. ) - Ain Shams University Faculty of Science Department of Biochemistry
 ( Khalil Wagdy K. B. ) - National Research Centre Cell Biology Department
 ( Salem Ahmed M. ) - Ain Shams University Faculty of Science Department of Biochemistry
 ( Farrag Abdel Razik H. ) - National Research Centre Pathology Department
 ( Ahmed Hanaa H. ) - Hormones Department, National Research Center, Dokki

Abstract


Purpose: Our research aims to address and determine the effect of Camellia sinensis extract in the management of nonalcoholic steatohepatitis (NASH) in rats.

Methods: Forty adult female albino rats were divided into four groups. Group 1 (G1) served as the control group, while the other three groups received high-fat diet for 32 weeks to induce NASH and then were later assigned to the following groups: (G2) NASH-afflicted group which was left untreated, (G3) NASH-afflicted group treated with Camellia sinensis extract in a dose of 400 mg/kg, and (G4) NASH-afflicted group treated with Camellia sinensis extract in a dose of 200 mg/kg.

Results: Significant elevation in serum alanine aminotransferase, albumin, bilirubin (total and direct), cholesterol, low density lipoprotein, triglycerides, leptin, Cox-2, and CD40 values was recorded. Moreover, overexpression of hepatic tumor necrosis factor alpha and hepatocyte growth factor genes were recorded, whereas blood platelet count and serum high density lipoprotein concentration revealed significant depletion, which was paralleled by significant downregulation of hepatic adiponectin gene expression level in NASH group versus the control group. On the opposite side, treatment of NASH groups with two different doses of Camellia sinensis extract reversed the values of the measured biochemical parameters and the targeted gene expression levels when compared with the NASH group. Optical micrograph of liver tissue sections of rats treated with Camellia sinensis extract showed the observed improvement in the studied biochemical and genetic markers.

Conclusion: This study provides a clear evidence for the promising therapeutic potential of Camellia sinensis extract against NASH. This could be ascribed to its hepatoprotective activity, hypolipidemic effect, and anti-inflammatory potency.

키워드

Nonalcoholic steatohepatitis; Camellia sinensis; Hyperlipidemia; Inflammation; Rats

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