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간 실질세포주에서 이호채(泥胡菜) 분획물이 liver X receptor α 의존적 지방 생성 유전자의 발현에 미치는 효과

The effects of Hemistepta lyrata Bunge (Bunge) fractionated extract on liver X receptor α-dependent lipogenic genes in hepatocyte-derived cells

대한한의학방제학회지 2020년 28권 3호 p.255 ~ 269
김재광, 조일제, 김은옥, 정대화, 구세광, 김상찬,
소속 상세정보
김재광 ( Kim Jae-Kwang ) - Daegu Haany University College of Korean Medicine
조일제 ( Cho Il-Je ) - Daegu Haany University College of Korean Medicine
김은옥 ( Kim Eun-Ok ) - Daegu Haany University College of Korean Medicine
정대화 ( Jung Dae-Hwa ) - Daegu Haany University College of Korean Medicine
구세광 ( Ku Sae-Kwang ) - Daegu Haany University College of Korean Medicine
김상찬 ( Kim Sang-Chan ) - Daegu Haany University College of Korean Medicine

Abstract


Objectives : Hemistepta lyrata Bunge (Bunge) is a wild herb that has been used for managing fever and wound in Korean Traditional Medicine. The present study explored the effects of H. lyrata extract on liver X receptor (LXR) α-dependent lipogenic genes in hepatocyte-derived cells.

Methods : After HepG2 cells or Huh7 cells were pre-treated with 1-10 μg/mL of H. lyrata extract or its fractionated extract for 0.5 h, the cells were subsequently exposed to LXR ligand for 6-24 h. Cell viability, LXR response element (LXRE)-driven luciferase activity, sterol regulatory element binding protein-response element (SREBP-RE)-driven luciferase activity, SREBP-1c expression, and mRNA levels of LXRα and its-dependent target genes were determined. In addition, LC-MS/MS analysis was conducted to explore major compounds in H. lyrata-chloroform fractionated extract #4 (HL-CF4).

Results : Of various H. lyrata extracts tested, chloroform extract and its fractionated extract #4, HL-CF4, significantly decreased T0901317-mediated SREBP-1c expression. In addition, HL-CF4 significantly reduced LXRE transactivation and LXRα mRNA expression without any cytotoxicity. Moreover, HL-CF4 prevented the SREBP-RE-driven luciferase activity and mRNA levels of fatty acid synthase and stearoyl-CoA desaturase-1 induced by T0901317. Results from LC-MS/MS analysis at positive/negative mode indicated that HL-CF4 contained several compounds showing m/z 197.1176 (C11H17O3), 693.2913/227.1069 (C38H45O12/C15H15O2), 203.1797 (C15H23), 181.1225 (C11H17O2), 591.2957 (C35H43O8), 379.1040 (C18H19O9), 409.1509 (C20H25O9), 309.1348 (C16H21O6), 391.1404 (C20H23O8), and 669.2924/389.1248 (C36H45O12/C20H21O8).

Conclusion : Based on its inhibition of the LXRα-dependent signaling pathway, H. lyrata chloroform extract and HL-CF4 have prophylactic potentials for managing non-alcoholic fatty liver.

키워드

Hemistepta lyrata chloroform fractionated extract; Hepatocyte-derived cell; Liver X receptor (LXR) α; Non-alcoholic fatty liver; T0901317

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