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제생신기환이 허혈-재관류로 유발된 급성 신부전 마우스에 미치는 효과

Effect of Jesaeng-sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mouse

대한한의학방제학회지 2021년 29권 1호 p.33 ~ 44
한병혁, 이현경, 장세훈, 태애림, 김혜윰, 이윤정, 이호섭, 강대길,
소속 상세정보
한병혁 ( Han Byung-Hyuk ) - Wonkwang University College of Korean Medicine
이현경 ( Lee Hyun-Kyung ) - Wonkwang University College of Korean Medicine
장세훈 ( Jang Se-Hoon ) - Wonkwang University College of Korean Medicine
태애림 ( Tai Ai-Lin ) - Wonkwang University College of Korean Medicine
김혜윰 ( Kim Hye-Yoom ) - Wonkwang University College of Korean Medicine
이윤정 ( Lee Yun-Jung ) - Wonkwang University College of Korean Medicine
이호섭 ( Lee Ho-Sub ) - Wonkwang University College of Korean Medicine
강대길 ( Kang Dae-Gill ) - Wonkwang University College of Korean Medicine

Abstract


Renal ischemia-reperfusion injury(IRI), an important cause of acute renal failure (ARF), cause increased renal tubular injury. Jesaeng-sinkihwan (JSH) was recorded in a traditional Chines medical book named “Bangyakhappyeon (方藥合編)”. JSH has been used for treatment of diabetes and glomerulonephritis with patients. Here we investigate the effects of Jesaeng-sinkihwan (JSH) in a mouse model of ischemic acute kidney injury. The animals model were divided into four groups at the age of 8 weeks; sham group: C57BL6 male mice (n=9), I/R group: C57BL6 male mice with I/R surgery (n=9), JSH Low group: C57BL6 male mice with surgery + JSH 100 mg/kg/day (n=9) and JSH High group: C57BL6 male mice with surgery + JSH 300 mg/kg/day (n=9). Ischemia was induced by clamping the both renal arteries during 25 min, and reperfusion was followed. Mouse were orally given with JSH (100 and 300 mg/kg/day during 3 days after surgery. Treatment with JSH significantly ameliorates creatinine clearance(Ccr), Creatinine (Cr) and blood urea nitrogen(BUN) in obtained plasma. . Treatment with JSH reduced kidney inflammation markers such as Neutrophil Gelatinase Associated Lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). JSH also reduced the periodic acid schiff (PAS) staining intensity and picro sirius red staining intensity in kidney of I/R group. These findings suggest that JSH ameliorates tubular injury including renal dysfunction in I/R induced ARF mouse.

키워드

Jesaeng-sinkihwan (JSH); Ischemia/Reperfusion; acute renal failure; creatinine clearance; renal-dysfunction; renal-fibrosis

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