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Dexamethasone으로 유도한 근위축 세포모델에서 흑효모 배양물 유래 polycan의 근위축 개선에 대한 효과

Effects of polysaccharide (polycan) derived from black yeast in dexamethasone-induced muscle atrophy cell model

대한한의학방제학회지 2021년 29권 1호 p.45 ~ 55
황수진, 임종민, 구본화, 이호섭, 정유진, 김영숙, 오태우,
소속 상세정보
황수진 ( Hwang Su-Jin ) - Daegu Haany University Department of Pharmaceutical Engineering
임종민 ( Lim Jong-Min ) - Glucan Co. Ltd.
구본화 ( Ku Bon-Hwa ) - Glucan Co. Ltd.
이호섭 ( Lee Ho-Sup ) - Glucan Co. Ltd.
정유진 ( Jung Yu-Jin ) - Korea Research Institute of Chemical Technology Research Center for Advanced Speciality Chemicals
김영숙 ( Kim Young-Suk ) - Glucan Co. Ltd.
오태우 ( Oh Tae-Woo ) - Korea Institute of Oriental Medicine Korean Medicine Application Center

Abstract


Objectives : This study was conducted to evaluate the anti-atrophic effect of polycan in dexamethasone-induced skeletal muscle atrophy in vitro model.

Methods : C2C12 myoblast were differentiated into myotube by 2% horese serum medium for 6 days, and then treated polycan extract at different concentrations for 24h. The effect of dexamethasone on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis.

Results : The results showed that Treatment with polycan (100 and 200 ㎍/㎖) noncytotoxic levels on both myoblast and myotube. Polycan decreased the ROS level overproduced with dexamethasone and improved the depletion of GSH level. Dexamethasone showed a decrease in myotube diameter, which was associated with up-regulation muscle-specific ubiquitin ligases markers, such as atrogin-1, FoxO3, myostatin and muscle RING finger-1 (MuRF1), and down-regulation of myogenin, MEF2, Myogenic regulatory factor 5, 6 and MyoD. The results showed that polycan treatment significantly dose-dependently inhibited it. Furthermore, decreased expressions of PI3K/Akt signal pathway by dexamethasone were reversed by treatment with polycan.

Conclusions : Thus, polycan suppresses dexamethasone induced muscle atrophy in C2C12 myotube in vitro model through activation of PI3K/Akt pathway and protective effect of improve skeletal muscle function.

키워드

Polycan; β-glucan; C2C12 myotubes; dexamethasone; anti-atrophic effect

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