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Determination of Diversity, Distribution and Host Specificity of Korean Laccaria Using Four Approaches

Mycobiology 2021년 49권 5호 p.461 ~ 468
조해진, 박기형, 박명수, 조윤희, 김지선, 서창완, 오승윤, 임영운,
소속 상세정보
조해진 ( Cho Hae-Jin ) - Seoul National University School of Biological Sciences
박기형 ( Park Ki-Hyeong ) - Seoul National University School of Biological Sciences
박명수 ( Park Myung-Soo ) - Seoul National University School of Biological Sciences
조윤희 ( Cho Yoon-Hee ) - Seoul National University School of Biological Sciences
김지선 ( Kim Ji-Seon ) - Seoul National University School of Biological Sciences
서창완 ( Seo Chang-Wan ) - Seoul National University School of Biological Sciences
오승윤 ( Oh Seung-Yoon ) - Changwon National University Department of Biology and Chemistry
임영운 ( Lim Young-Woon ) - Seoul National University School of Biological Sciences

Abstract


The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.

키워드

Ectomycorrhizal fungi; NGS; Pinus densiflora; Quercus mongolica; speciesspecific primer

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