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Anti-Fatigue Activity of a Mixture of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. Fruit Extract

Preventive Nutrition and Food Science 2020년 25권 4호 p.380 ~ 388
오주현, 한윤영, 김지민, 박찬성, 오둘리, 윤효정, 이규옥, 김재용, 최철웅, 이용욱,
소속 상세정보
오주현 ( Oh Joo-Hyun ) - Naturalendo Tech Co. Ltd. Herbal Hormone Research Institute
한윤영 ( Han Yoon-Young ) - Naturalendo Tech Co. Ltd. Herbal Hormone Research Institute
김지민 ( Kim Ji-Min ) - Naturalendo Tech Co. Ltd. Herbal Hormone Research Institute
박찬성 ( Park Chan-Sung ) - Naturalendo Tech Co. Ltd. Herbal Hormone Research Institute
오둘리 ( Oh Dool-Ri ) - Jeollanamdo Institute of Natural Resources Research
윤효정 ( Yun Hyo-Jeong ) - Jeollanamdo Institute of Natural Resources Research
이규옥 ( Lee Gyu-Ok ) - Jeollanamdo Institute of Natural Resources Research
김재용 ( Kim Jae-Yong ) - Jeollanamdo Institute of Natural Resources Research
최철웅 ( Choi Chul-Yung ) - Jeollanamdo Institute of Natural Resources Research
이용욱 ( Lee Yong-Wook ) - Naturalendo Tech Co. Ltd. Herbal Hormone Research Institute

Abstract


Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. are commonly used in traditional herbal medicine and food and both exhibit antioxidant and anti-inflammatory effects. Herein, hot-water extracts of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. fruits (1:1 mixture) were used to produce a complex extract NET-1601. The anti-fatigue activity of NET-1601 was evaluated in an in vitro oxidative stress model induced by treating C2C12 myotubes with H2O2. An exhaustive swimming test (EST) in vivo model was established using ICR mice. NET-1601-treated C2C12 myotubes (50, 100, and 200 μg/mL) with H2O2-induced oxidative stress displayed significantly increased cell viability and ATP content, but significantly decreased levels of reactive oxygen species. All NET-1601-treated EST models demonstrated significantly higher maximum swimming rates than control mice. Furthermore, serum lactate, lactate dehydrogenase activity, non-esterified fatty acid, and intramuscular glycogen levels were higher in NET-1601- treated mice than in control mice. In addition, mRNA levels of regulatory factors involved in muscle mitochondrial fatty acid β-oxidation increased upon NET-1601 treatment. Moreover, catalase, superoxide dismutase, glutathione-S-transferase, and liver glutathione content, and antioxidant activity were higher in NET-1601-treated mice than in control mice. Reduced malondialdehyde levels indicated that NET-1601 treatment inhibited exercise-induced lipid peroxidation. Together, these results suggest that NET-1601 retains antioxidant enzyme activity during oxidative stress, simultaneously enhancing both muscle function via glycogen and fatty acid oxidation, thereby exerting a positive effect on recovery from fatigue.

키워드

anti-fatigue activity; exhaustive swimming test; oxidative stress; Stauntonia hexaphylla (Thunb.) Decaisne; Vaccinium bracteatum Thunb.

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