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Synthetic High-Density Lipoprotein-Like Nanocarrier Improved Cellular Transport of Lysosomal Cholesterol in Human Sterol Carrier Protein-Deficient Fibroblasts

Journal of Medicinal Food 2016년 19권 1호 p.62 ~ 67
남다은, 김옥경, 박유경, 이정민,
소속 상세정보
남다은 ( Nam Da-Eun ) - Kyung Hee University Department of Medical Nutrition
김옥경 ( Kim Ok-Kyung ) - Kyung Hee University Department of Medical Nutrition
박유경 ( Park Yoo-Kyoung ) - Kyung Hee University Department of Medical Nutrition
이정민 ( Lee Jeong-Min ) - Kyung Hee University Department of Medical Nutrition

Abstract


Sterol carrier protein-2 (SCP-2), which is not found in tissues of people with Zellweger syndrome, facilitates the movement of cholesterol within cells, resulting in abnormal accumulation of cholesterol in SCP-2-deficient cells. This study investigated whether synthetic high-density lipoprotein-like nanocarrier (sHDL-NC) improves the cellular transport of lysosomal cholesterol to plasma membrane in SCP-2-deficient fibroblasts. Human SCP-2-deficient fibroblasts were incubated with [3H-cholesterol]LDL as a source of cholesterol and sHDL-NC. The cells were fractionated by centrifugation permit tracking of [3H]-cholesterol from lysosome into plasma membrane. Furthermore, cellular content of cholesteryl ester as a storage form and mRNA expression of low-density lipoprotein (LDL) receptor were measured to support the cholesterol transport to plasma membrane. Incubation with sHDL-NC for 8?h significantly increased uptake of [3H]-cholesterol to lysosome by 53% and further enhanced the transport of [3H]-cholesterol to plasma membrane by 32%. Treatment with sHDL-NC significantly reduced cellular content of cholesteryl ester and increased mRNA expression of LDL receptor (LDL-R). In conclusion, sHDL-NC enables increased transport of lysosomal cholesterol to plasma membrane. In addition, these data were indirectly supported by decreased cellular content of cholesteryl ester and increased gene expression of LDL-R. Therefore, sHDL-NC may be a useful vehicle for transporting cholesterol, which may help to prevent accumulation of cholesterol in SCP-2-deficient fibroblasts.

키워드

cholesterol; LDL receptor; SCP-2; sHDL-NC

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