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Fisetin-Rich Extracts of Rhus verniciflua Stokes Improve Blood Flow Rates in Mice Fed Both Normal and High-Fat Diets

Journal of Medicinal Food 2016년 19권 2호 p.120 ~ 126
임원균, 박현정, 이광수, 이정훈, 김영동, 김경희, 박상재, 홍석만, 전성호,
소속 상세정보
임원균 ( Im Won-Kyun ) - Hallym University Department of Life Science
박현정 ( Park Hyun-Jung ) - Sejong University Department of Bioscience and Biotechnology
이광수 ( Lee Kwang-Soo ) - Hallym University Department of Life Science
이정훈 ( Lee Jung-Hoon ) - Hallym University Department of Life Science
김영동 ( Kim Young-Dong ) - Hallym University Department of Life Science
김경희 ( Kim Kyeong-Hee ) - Medience Co. Ltd.
박상재 ( Park Sang-Jae ) - Medience Co. Ltd.
홍석만 ( Hong Seok-Mann ) - Sejong University Department of Bioscience and Biotechnology
전성호 ( Jeon Sung-Ho ) - Hallym University Department of Life Science

Abstract


Although it has been previously reported that Rhus verniciflua Stokes (RVS) possesses in vitro anti-inflammatory activity, the precise in vivo mechanisms of RVS extracts and a main active component called fisetin have not been well elucidated. In this study, using newly developed protocols, we prepared urushiol-free but fisetin-enriched RVS extracts and investigated their effects on the vascular immune system. We found that the water-soluble fractions of detoxified RVS with the flavonoid fisetin can inhibit lipopolysaccharide-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2). Furthermore, RVS can reduce inducible nitric oxide synthase and COX2 gene expression levels, which are responsible for NO and PGE2 production, respectively, in RAW264.7 macrophage cells. Because inflammation is linked to the activation of the coagulation system, we hypothesized that RVS and its active component fisetin possess anticoagulatory activities. As expected, we found that both RVS and fisetin could inhibit the coagulation of human peripheral blood cells. Moreover, in vivo RVS treatment could return the retarded blood flow elicited by a high-fat diet (HFD) back to the normal level in mice. In addition, RVS treatment has significantly reduced body weight gained by HFD in mice. Taken together, the fisetin-rich RVS extracts have potential antiplatelet and antiobesity activities and could be used as a functional food ingredient to improve blood circulation.

키워드

anticoagulatory; anti-inflammatory; antiobesity; fisetin; Health functional food; Rhus verniciflua

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