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In Vitro and In Vivo Immunomodulatory Activity of Okra (Abelmoschus esculentus L.) Polysaccharides

Journal of Medicinal Food 2016년 19권 3호 p.253 ~ 265
Chen Huricha, Jiao Hanwei, Cheng Ying, Xu Kailian, Jia Xiaoxiao, Shi Qiaoyun, Guo Shiyu, Wang Manchuriga, Du Li, Wang Fengyang,
소속 상세정보
 ( Chen Huricha ) - Hainan University College of Agriculture
 ( Jiao Hanwei ) - Hainan University College of Agriculture
 ( Cheng Ying ) - Hainan University College of Agriculture
 ( Xu Kailian ) - Hainan University College of Agriculture
 ( Jia Xiaoxiao ) - Hainan University College of Agriculture
 ( Shi Qiaoyun ) - Hainan University College of Agriculture
 ( Guo Shiyu ) - Hainan University College of Agriculture
 ( Wang Manchuriga ) - Hainan University College of Agriculture
 ( Du Li ) - Hainan University College of Agriculture
 ( Wang Fengyang ) - Hainan University College of Agriculture

Abstract


Crude okra (Abelmoschus esculentus L.) polysaccharide (RPS) was obtained by water extraction and alcohol precipitation. Three purified fractions of RPS, designated RPS-1, RPS-2, and RPS-3, were fractioned by diethylaminoethyl (DEAE)-cellulose chromatography. Their molecular weights, monosaccharide compositions, infrared (Fourier transform infrared [FT-IR]) spectra, and nuclear magnetic resonance (NMR) spectra were analyzed. Their immunomodulatory activity was evaluated with an in vitro cell model (RAW264.7 cells). In vivo immunomodulatory activity of RPS-2 was evaluated in normal and cyclophosphamide-induced immunosuppressed mice. The results showed that the molecular weights of RPS-1, RPS-2, and RPS-3 were 600, 990, and 1300?kDa, respectively. RPS-1 and RPS-2 were mainly composed of galactose, rhamnose, galacturonic acid, and glucuronic acid, while RPS-3 was mainly composed of galactose, rhamnose, galacturonic acid, glucuronic acid, and glucose. FT-IR and NMR spectrum data indicated a rhamnogalacturonan I characteristic of polysaccharide. Both RPS and its purified fractions RPS-1, RPS-2, and RPS-3 significantly increased RAW264.7 cell proliferation, nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) expression, and tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-10 secretion (P?

키워드

cytokine; nitric oxide; okra (Abelmoschus esculentus L.); polysaccharide; RAW264.7

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