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Osteoprotective Effects of Polysaccharide-Enriched Hizikia fusiforme Processing Byproduct In Vitro and In Vivo Models

Journal of Medicinal Food 2016년 19권 8호 p.805 ~ 814
정용태, 백승화, 정상철, 윤여대, 김옥희, 오병철, 정지욱, 김진희,
소속 상세정보
정용태 ( Jeong Yong-Tae ) - Daegu Haany University
백승화 ( Baek Seung-Hwa ) - Kyungpook National University Department of Food Science and Biotechnology
정상철 ( Jeong Sang-Chul ) - Nakdonggang National Institute of Biological Resources Freshwater Bioresources Utilization Division
윤여대 ( Yoon Yeo-Dae ) - Korea Research Institute of Bioscience and Biotechnology
김옥희 ( Kim Ok-Hee ) - Gachon University Graduate School of Medicine Lee Gil Ya Cancer and Diabetes Institute
오병철 ( Oh Byung-Chul ) - Gachon University Graduate School of Medicine Lee Gil Ya Cancer and Diabetes Institute
정지욱 ( Jung Ji-Wook ) - Daegu Haany University Department of Natural Cosmetic Ingredient
김진희 ( Kim Jin-Hee ) - Daegu Haany University College of Herbal Bio-Industry

Abstract


The traditional manufacturing method used to produce goods from Hizikia fusiforme, utilizes extraction steps with hot water. The byproduct (of hot water extraction) is rich in polysaccharide and is considered a waste. To evaluate the osteogenic effects of the byproduct of H. fusiforme (HFB), osteogenic cells and animal models were used to test it effects on osteogenesis. The HFB-treated mouse myoblast C2C12 cells exhibited significant dose dependently elevated alkaline phosphatase (ALP) activity and slightly increased bone morphogenetic protein-2 (BMP-2). HFB also suppressed the formation of tartrate-resistant acid phosphatase (TRAP) activity and TRAP staining in the bone marrow-derived macrophages (BMM) cells that had been stimulated with the receptor activator of the nuclear factor kB ligand/macrophage colony-stimulating factor kB ligand. In addition, HFB also increased the phosphorylation of extracellular signal-regulated protein kinase (p-ERK) level. Finally, osteogenic effects of HFB were clearly confirmed in the three in vivo models: zebrafish, ovariectomized mice, and mouse calvarial bones. HFB accelerated the rate of skeletal development in zebrafish and prevented much of the mouse femoral bone density loss of ovariectomized mice. Moreover, HFB enhanced woven bone formation over the periosteum of mouse calvarial bones. Our result showed that HFB functions as a bone resorption inhibitor as well as an activator of bone formation in vivo and in osteogenic in vitro cell systems.

키워드

Hizikia fusiforme; osteoblast; osteoclast; ovariectomized mice; zebrafish

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