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Anti-Inflammatory Effect of Ethanolic Extract of Sargassum serratifolium in Lipopolysaccharide-Stimulated BV2 Microglial Cells

Journal of Medicinal Food 2016년 19권 11호 p.1023 ~ 1031
오선지, 정은지, 권미성, 이봉기, Utsuki Tadanobu, 오철웅, 김형락,
소속 상세정보
오선지 ( Oh Sun-Ji ) - Pukyoung National University Department of Food and Nutrition
정은지 ( Joung Eun-Ji ) - Pukyoung National University Department of Food and Nutrition
권미성 ( Kwon Mi-Sung ) - Pukyoung National University Department of Food and Nutrition
이봉기 ( Lee Bong-Gi ) - Pusan National University College of Pharmacy
 ( Utsuki Tadanobu ) - Louisiana State University School of Veterinary Medicine Department of Pathobiological Science
오철웅 ( Oh Chul-Woong ) - Pukyoung National University Department of Marine Biology
김형락 ( Kim Hyeung-Rak ) - Pukyoung National University Department of Food and Nutrition

Abstract


Sargassum serratifolium was found to contain high concentrations of meroterpenoids, having strong antioxidant, anti-inflammatory, and neuroprotective activities. This study aims to investigate the anti-inflammatory mechanisms of an ethanolic extract of S. serratifolium (ESS) using lipopolysaccharide (LPS)-stimulated BV2 microglial cells and to identify the anti-inflammatory components in ESS. The level of proinflammatory cytokines was measured by enzyme-linked immunosorbent assay. The expression of inflammation-related proteins and mRNA was evaluated by Western blot and reverse transcription-polymerase chain reaction analysis, respectively. Anti-inflammatory activities of isolated components from ESS were analyzed in LPS-stimulated BV2 cells. ESS inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 and the expression of inducible NO synthase and cyclooxygenase-2. ESS also decreased the release of proinflammatory cytokines in a dose-dependent manner. LPS-induced nuclear factor-kappa B (κB) transcriptional activity and translocation into the nucleus were remarkably suppressed by ESS through the prevention of inhibitor κB-α degradation. The main anti-inflammatory components in ESS were identified as sargahydroquinoic acid, sargachromenol, and sargaquinoic acid based on the inhibition of NO production using LPS-stimulated BV2 cells. Furthermore, treatment with ESS significantly reduced levels of tumor necrosis factor-α and interleukin-1β stimulated with LPS in mouse hippocampus. Our results indicate that ESS can be used as a functional food or therapeutic agent for the treatment of neuroinflammatory diseases.

키워드

anti-inflammation; sargachromenol; sargahydroquinoic acid; sargaquinoic acid; Sargassum serratifolium

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