잠시만 기다려 주세요. 로딩중입니다.

Apigetrin from Scutellaria baicalensis Georgi Inhibits Neuroinflammation in BV-2 Microglia and Exerts Neuroprotective Effect in HT22 Hippocampal Cells

Journal of Medicinal Food 2016년 19권 11호 p.1032 ~ 1040
임혜선, 김온순, 김부여, 정수진,
소속 상세정보
임혜선 ( Lim Hye-Sun ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division
김온순 ( Kim Ohn-Soon ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division
김부여 ( Kim Bu-Yeo ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division
정수진 ( Jeong Soo-Jin ) - Korea Institute of Oriental Medicine Herbal Medicine Research Division

Abstract


Apigetrin is a flavonoid isolated from various herbal medicines such as Scutellaria baicalensis Georgi, Matricaria chamomilla, Stachys tibetica Vatke, and Teucrium gnaphalodes. In the present study, we investigated the inhibitory effects of apigetrin on neuroinflammation using the BV-2 microglia cell line. Our data revealed that apigetrin significantly reduced secretion and mRNA expression of inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), in lipopolysaccharide (LPS)-stimulated BV-2 mouse microglia. Apigetrin also significantly decreased LPS-mediated production of prostaglandin E2 (PGE2) level and nitric oxide (NO) production as well as expression of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) in BV-2 cells. In addition, apigetrin suppressed nuclear expression of nuclear factor kappa B (NF-κB) in LPS-stimulated BV-2 cells. Furthermore, apigetrin significantly impaired reactive oxygen species (ROS) generation and enhanced expression of antioxidant enzymes, hempxygenase 1 (HO-1) and nuclear factor-like 2 (Nrf2), in BV-2 cells. Apigetrin also increased 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, indicating antioxidative activity of apigetrin. Moreover, we found that apigetrin inhibited hydrogen peroxide (H2O2)-induced cell death in HT22 hippocampal cells. Overall, our findings indicate that apigetrin has inhibitory effects on neuroinflammation as well as antioxidation and neuroprotection, suggesting the potential prophylactic activity for neurodegenerative diseases through the inter-regulation of neuroinflammation, oxidative stress, and neuronal injury.

키워드

apigetrin; anti-neuroinflammation; Scutellaria baicalensis; neuroprotection

원문 및 링크아웃 정보

등재저널 정보