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Gynostemma pentaphyllum Ethanolic Extract Protects Against Memory Deficits in an MPTP-Lesioned Mouse Model of Parkinson's Disease Treated with L-DOPA

Journal of Medicinal Food 2017년 20권 1호 p.11 ~ 18
김경수, Zhao Ting Ting, Shin Keon-Sung, 박현진, 조윤정, 이경은, 김승환, 이명구,
소속 상세정보
김경수 ( Kim Kyung-Sook ) - Chungbuk National University College of Pharmacy Department of Pharmacy
 ( Zhao Ting Ting ) - Chungbuk National University College of Pharmacy Department of Pharmacy
 ( Shin Keon-Sung ) - Chungbuk National University College of Pharmacy Department of Pharmacy
박현진 ( Park Hyun-Jin ) - Chungbuk National University College of Pharmacy Department of Pharmacy
조윤정 ( Cho Yoon-Jeong ) - Chungbuk National University College of Pharmacy Department of Pharmacy
이경은 ( Lee Kyung-Eun ) - Chungbuk National University College of Pharmacy Department of Pharmacy
김승환 ( Kim Seung-Hwan ) - Songwon University Department of Social Physical Education
이명구 ( Lee Myung-Koo ) - Chungbuk National University College of Pharmacy Department of Pharmacy

Abstract


This study investigated the effects of ethanol extract from Gynostemma pentaphyllum (GP-EX) on memory deficits in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned mouse model of Parkinson's disease (PD) (MPTP-lesioned mice). MPTP (30?mg/kg/day, 5 days)-lesioned mice showed deficits of habit learning memory and spatial memory, which were further aggravated by treatment with L-3,4-dihydroxyphenylalanine (L-DOPA) (25?mg/kg, 21 days). However, treatment with GP-EX (50?mg/kg, 21 days) ameliorated memory deficits in MPTP-lesioned mice treated with L-DOPA (25?mg/kg): GP-EX prevented the decreases in retention latency time in the passive avoidance test and tyrosine hydroxylase-immunopositive cells and dopamine levels in the nigrostriatum. GP-EX also reduced increases in retention transfer latency time of the elevated plus-maze test and expression of N-methyl-D-aspartate (NMDA) receptor and improved decreases in phosphorylation of extracellular signal-regulated kinase (ERK1/2) and cyclic AMP-response element binding protein (CREB) in the hippocampus in the same models. By contrast, L-DOPA treatment (10?mg/kg, 21 days) ameliorated memory deficits in MPTP-lesioned mice, which were further improved by GP-EX treatment. These results suggest that GP-EX ameliorates habit learning memory deficits by activating dopaminergic neurons and spatial memory deficits by modulating NMDA receptor-ERK1/2-CREB system in MPTP-lesioned mice treated with L-DOPA. GP-EX may serve as an adjuvant phytonutrient for memory deficits in PD.

키워드

Gynostemma pentaphyllum; L-DOPA; memory deficits; MPTP-lesioned mouse; Parkinson's disease

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