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Neuroprotective Effect of Halophyte Salicornia herbacea L. Is Mediated by Activation of Heme Oxygenase-1 in Mouse Hippocampal HT22 Cells

Journal of Medicinal Food 2017년 20권 2호 p.140 ~ 151
김민선, 서지연, 오지선, 장유경, 이충환, 김종상,
소속 상세정보
김민선 ( Kim Min-Sun ) - Kyungpook National University School of Food Science and Biotechnology
서지연 ( Seo Ji-Yeon ) - Kyungpook National University School of Food Science and Biotechnology
오지선 ( Oh Ji-Sun ) - Kyungpook National University School of Food Science and Biotechnology
장유경 ( Jang Yu-Kyung ) - Konkuk University Department of Bioscience and Biotechnology
이충환 ( Lee Choong-Hwan ) - Konkuk University Department of Bioscience and Biotechnology
김종상 ( Kim Jong-Sang ) - Kyungpook National University School of Food Science and Biotechnology

Abstract


Salicornia herbacea L. (glasswort, tungtungmadi in Korean), a halophyte that grows in salt marshes and muddy seashores along the western coast of Korea, has been used as a seasoning vegetable and a folk medicine for intestinal ailments, nephropathy, and hepatitis. As the salt-tolerant herb was reported to contain antioxidants, including tungtungmadic acid, quercetin, and chlorogenic acid, we hypothesized that the ethanolic extract of S. herbacea L. (SH extract) enriched with antioxidative compounds will have neuroprotective activity. The herbal extract and its methylene chloride (MC) fraction showed a strong protective effect against glutamate-induced cell death in murine hippocampal HT22 cells. In addition, SH extract and MC fraction not only scavenged reactive oxygen species efficiently but also caused nuclear translocation of nuclear factor (erythroid-derived 2)-like 2 and subsequently significant induction of antioxidant enzymes such as NAD(P)H:quinone oxidoreductase, heme oxygenase 1 (HO-1), and glutathione reductase. Inhibition of the antioxidant enzyme HO-1 by tin protoporphyrin abolished the neuroprotective effect of the SH extract, suggesting an important role of HO-1 in protection against glutamate-induced neural damage. Metabolite profiling for ethanolic extract and solvent fractions of the herb suggested that diosmetin and a few unidentified compounds were responsible for the neuroprotective effect. Taken together, SH extract and its MC fraction exhibited a neuroprotective effect through Nrf2-mediated induction of antioxidant enzymes, such as HO-1, and warrant further in vivo and clinical studies to confirm its effects and potentially develop a neuroprotective salt substitute or dietary supplement.

키워드

diosmetin; HO-1; neuroprotection; Nrf2; Salicornia herbacea L.

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