잠시만 기다려 주세요. 로딩중입니다.

Time Course Effect of R-Alpha-Lipoic Acid on Cellular Metabolomics in Cultured Hepatoma Cells

Journal of Medicinal Food 2017년 20권 3호 p.211 ~ 222
Ikuta Naoko, Chikamoto Keita, Asano Yuya, Yasui Yoshiaki, Yokokawa Haruka, Terao Keiji, Rimbach Gerald, Matsugo Seiichi,
소속 상세정보
 ( Ikuta Naoko ) - Kobe University Graduate School of Medicine
 ( Chikamoto Keita ) - Kanazawa University College of Science and Engineering
 ( Asano Yuya ) - Kanazawa University College of Science and Engineering
 ( Yasui Yoshiaki ) - Kanazawa University College of Science and Engineering
 ( Yokokawa Haruka ) - Kanazawa University College of Science and Engineering
 ( Terao Keiji ) - CycloChem Bio Co. Ltd.
 ( Rimbach Gerald ) - University of Kiel Institute of Human Nutrition and Food Science
 ( Matsugo Seiichi ) - Kanazawa University College of Science and Engineering

Abstract


Alpha-lipoic acid (LA) is a powerful antioxidant. LA has two enantiomers, R(+)-LA (R-LA) and S(?)-LA (S-LA). Of these, R-LA is naturally occurring and an essential cofactor in energy metabolism. R-LA treatment has been reported to affect glucose metabolism in rat hepatoma cells. This study analyzed the time course of metabolite levels in LA-treated cultured H4IIEC3 rat hepatoma cells, including a specific evaluation of the effect of R-LA and the enantioselectivity of LA. Principal component analysis showed that this experiment was well designed to observe enantioselectivity. R-LA treatment was found to inhibit the glycolysis and Thr-Gly-Ser pathways, as well as lactic acid production, leading to the inhibition of gluconeogenesis in starved H4IIEC3 cells. This study may provide mechanistic insight into how R-LA induces apoptosis in hepatoma cells.

키워드

amino acids; enantioselectivity; glyconeogenesis; hepatoma cells; H4IIEC3; lipoic acid; metabolomics

원문 및 링크아웃 정보

등재저널 정보