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Transport of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide Ala-His-Leu-Leu Across Human Intestinal Epithelial Caco-2 Cells

Journal of Medicinal Food 2017년 20권 3호 p.243 ~ 250
Li Ying, Zhao Jiangtao, Liu Xiaoli, Xia Xiudong, Wang Ying, Zhou Jianzhong,
소속 상세정보
 ( Li Ying ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing
 ( Zhao Jiangtao ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing
 ( Liu Xiaoli ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing
 ( Xia Xiudong ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing
 ( Wang Ying ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing
 ( Zhou Jianzhong ) - Jiangsu Academy of Agricultural Sciences Institute of Agricultural Products Processing

Abstract


The transport behavior and absorption mechanism of Ala-His-Leu-Leu (AHLL) intestinal absorption in Caco-2 cell monolayers were clarified systemically. The safe absorptive concentration of AHLL was 200?μg/mL, which was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The permeation of AHLL was concentration dependent in a bidirectional transfer and reached a plateau at 90?min. The efflux ratio was above 0.5, suggesting that AHLL was absorbed by both active transport and passive diffusion. The apparent permeability coefficients (Papp) of AHLL both from the apical (AP) to basolateral (BL) side (PappAB) and from the BL to AP side (PappBA) decreased when the temperature was lowered from 37°C to 4°C.The uptake of AHLL was more at pH 7.4 than at other pHs. Both verapamil and (E)-3-[[[3-[2-(7-chloro-2- quinolinyl) ethenyl] phenyl]-[[(3-dimethyl amino)-3-oxopropyl]thio] methyl] thio]-propanoic acid (MK571) inhibited the absorption of AHLL, indicating that P-glycoprotein and multi-drug resistant proteins (MRPs) were all involved in AHLL secretion, especially multi-drug resistant protein 2 (MRP2). AHLL was transported through both trans- and paracellular pathways across the Caco-2 cell monolayer. This work first elucidates the AHLL absorption mechanism in Caco-2 cells and provides the basis for future studies on the improvement of bioavailability.

키워드

Ala-His-Leu-Leu; Caco-2 cell monolayer; transport; intestinal absorption mechanism; antihypertensive peptide

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