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Blood Pressure-Lowering Effects of Alacalase-Hydrolyzed Camellia Seed Hull In Vitro and in Spontaneous Hypertensive Rats

Journal of Medicinal Food 2017년 20권 7호 p.720 ~ 723
임호정, 김미소, 김다솜, 김영준, 이진협, 판정훈, 신의철, 김재겸,
소속 상세정보
임호정 ( Lim Ho-Jeong ) - Gyeongnam National University of Science and Technology Department of Food Science
김미소 ( Kim Mi-So ) - Gyeongnam National University of Science and Technology Department of Food Science
김다솜 ( Kim Da-Som ) - Gyeongnam National University of Science and Technology Department of Food Science
김영준 ( Kim Young-Jun ) - korea university Department of Food and Biotechnology
이진협 ( Lee Jin-Hyup ) - korea university Department of Food and Biotechnology
판정훈 ( Pan Jeong-Hoon ) - University of Arkansas School of Human Environmental Sciences
신의철 ( Shin Eui-Cheol ) - Gyeongnam National University of Science and Technology Department of Food Science
김재겸 ( Kim Jae-Kyeom ) - University of Arkansas School of Human Environmental Sciences

Abstract


High blood pressure is one of the major risk factors for various diseases and angiotensin-converting enzyme (ACE) plays a critical role in blood pressure regulation. In our study, the responsive surface methodology was adopted to establish optimal Alcalase-hydrolysis conditions of camellia seed hull against ACE activity. The optimum conditions are hydrolysis temperature of 50.98°C, enzyme/substrate ratio of 2.85%, and hydrolysis pH of 7.12. In an animal feeding study, spontaneously hypertensive rats were treated with either a low or high dose of hydrolyzed Camellia japonica seed cake over 5 weeks. Even though systolic blood pressure was not statistically different, the high dose of C. japonica hydrolysate lowered diastolic blood pressure (106?±?4.4?mmHg vs. 145?±?5.9?mmHg) at the 5th week. A similar trend was also observed in serum ACE activity. Considering that this camellia seed hull is a major resource of this plant, our study provides important data to utilize this plant for both academic and industrial applications.

키워드

alcalase; angiotensin-converting enzyme; antihypertensive effect; Camellia japonica; spontaneously hypertensive rat

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