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Chemical Composition and Antimigraine Activity of Essential Oil of Angelicae dahuricae Radix

Journal of Medicinal Food 2017년 20권 8호 p.797 ~ 803
Sun Jingbo, Li He, Sun Jinghui, Liu Huimin, Chen Jianguang, Wang Chunmei,
소속 상세정보
 ( Sun Jingbo ) - Beihua University Department of Medicinal Chemistry
 ( Li He ) - Beihua University College of Pharmacy Department of Pharmacology
 ( Sun Jinghui ) - Beihua University College of Pharmacy Department of Pharmacology
 ( Liu Huimin ) - Beihua University Affiliated Hospital Department of Neurology
 ( Chen Jianguang ) - Beihua University College of Pharmacy Department of Pharmacology
 ( Wang Chunmei ) - Beihua University College of Pharmacy Department of Pharmacology

Abstract


The aim of this study was to explore the chemical composition and the effect of essential oil of Angelicae dahuricae radix on a nitroglycerin (NTG)-induced rat model of migraine. The CO2 supercritical fluid extraction method was optimized for the extraction of essential oil of A. dahuricae radix (EOAD) and its chemical composition was determined. The migraine model was established by subcutaneous injection of NTG (10?mg/kg) 1?h after the last administration of EOAD. The therapeutic effect of EOAD and its underlying mechanism were assessed by monitoring behavioral changes, levels of nitric oxide (NO) in serum and brain tissues, plasma levels of calcitonin gene-related peptide (CGRP) and endothelin (ET), and ET/NO ratio. The optimal conditions for CO2 supercritical fluid extraction of EOAD, as determined by orthogonal test [L9(34)], were as follows: 2?h extraction time, 20?MPa pressure, 40°C temperature, and 30 mesh. The yield of EOAD was 1.8%. On gas chromatography?mass spectrometry, 45 peaks were found in EOAD, and 22 compounds were identified and quantified. The main constituents of EOAD were 1-dodecanol (13.71%), elemene (7.54%), palmitic acid ethyl ester (7.32%), α-pinene (6.25%), and 1-pentadecanol (6.08%). Compared with rat migraine model controls, EOAD (35, 70, and 140?mg/kg) significantly reduced the number of head shaking, head scratching, and hind leg shooting events, decreased serum and brain NO levels, decreased plasma CGRP, and increased ET levels in rats. ET/NO ratio was elevated to 28.68 in the EOAD high-dose group. EOAD ameliorates NTG-induced migraine in rats likely by modulating the levels of vasoactive substances.

키워드

Angelicae dahuricae radix; calcitonin gene-related peptide; endothelin; essential oil; migraine disorders; nitric oxide

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