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Artemisia dracunculus Extracts Obtained by Organic Solvents and Supercritical CO2 Produce Cytotoxic and Antitumor Effects in Mice with L5178Y Lymphoma

Journal of Medicinal Food 2017년 20권 11호 p.1076 ~ 1082
Navarro-Salcedo Martha Hilda, Delgado-Saucedo Jorge Ivan, Siordia-Sanchez Victor Hugo, Gonzalez-Ortiz Luis J., Castillo-Herrera Gustavo Adolfo, Puebla-Perez Ana M.,
소속 상세정보
 ( Navarro-Salcedo Martha Hilda ) - University of Guadalajara University Center of Exact Sciences and Engineering Pharmacobiology Department
 ( Delgado-Saucedo Jorge Ivan ) - University of Guadalajara University Center of Exact Sciences and Engineering Pharmacobiology Department
 ( Siordia-Sanchez Victor Hugo ) - University of Guadalajara University Center of Exact Sciences and Engineering Pharmacobiology Department
 ( Gonzalez-Ortiz Luis J. ) - University of Guadalajara University Center of Exact Sciences and Engineering Chemistry Department
 ( Castillo-Herrera Gustavo Adolfo ) - Center for Research and Assistance in Technology and Design of the State of Jalisco Department of Food Technology
 ( Puebla-Perez Ana M. ) - University of Guadalajara University Center of Exact Sciences and Engineering Pharmacobiology Department

Abstract


We investigated the cytotoxic and antitumor effects of nine leaf extracts from Artemisia dracunculus (Tarragon). Five extracts were obtained using different organic solvents and four by supercritical CO2. The cytotoxic effects were expressed as IC50 in 100, 80, 80, 100, and 80?μg/mL by respective solvents: hexane, ethyl acetate, acetone, ethanol, and acetonitrile in L5178Y lymphoma cells. For supercritical CO2 extract A, IC50 was 100?μg/mL; for extracts C and D, IC50 was 150?μg/mL. The antitumor activity was assessed through a tumor growth inhibition test that measured ascites fluid volume and tumor cell counts of BALB/c mice (2?×?104 cells L5178Y i.p.). Twenty-four hours after inoculation, mice were treated with 100?mg/kg of acetonitrile extract or extract SF-A daily for 15 days in independent groups of five mice, using two administration routes. We observed tumor evolution with and without treatment. Without treatment, tumor evolution was 17,969?×?106?±?5485 L5178Y cells in 2.6?mL ascites volume, whereas the orally treated acetonitrile extract group showed 0.1?×?106?±?0.07 L5178Y cells (P?

키워드

antitumor activity;?Artemisia dracunculus;?extract organic solvent;?extract supercritical CO2

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