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Chlorogenic Acid-Enriched Extract of Ilex kudingcha C.J. Tseng Inhibits Angiogenesis in Zebrafish

Journal of Medicinal Food 2017년 20권 12호 p.1160 ~ 1167
Zhong Tao, Piao Linghua, 김현정, Liu Xiande, Jiang Shengnan, Liu Guomin,
소속 상세정보
 ( Zhong Tao ) - Hainan University Department of Veterinary Medicine
 ( Piao Linghua ) - Hainan Medical University Department of Physiology
김현정 ( Kim Hyun-Jung ) - Mokpo National University College of Pharmacy
 ( Liu Xiande ) - Hainan University Department of Veterinary Medicine
 ( Jiang Shengnan ) - Central South University Xiangya School of Medicine Haikou Hospital Department of Nuclear Medicine
 ( Liu Guomin ) - Hainan University Kudingcha Institute

Abstract


Kudingcha is a particularly bitter tasting tea that has been widely used in China to eliminate fever and itching eyes, and to clear blood toxins. Kudingcha is considered of value for its potential anticancer effects that are attributed to the presence of characteristic bioactive ingredients. The chlorogenic acid (CGA) derivatives 3-0-caffeoylquinic acid, 5-0-caffeoylquinic acid, 3,5-0-dicaffeoylquinic acid, and 4,5-0-dicaffeoylquinic acid were separated from Ilex kudingcha C.J. Tseng extract by high-performance liquid chromatography (HPLC)?photodiode array detector (PDA) and HPLC-nuclear magnetic resonance (NMR). In Tg(flk1:EGFP) zebrafish embryos at 52 hours postfertilization (hpf), angiogenesis was significantly inhibited by kudingcha extract (KDCE) at concentrations of 400 and 500?μg/mL and CGA also showed significant inhibition in embryos treated with 80, 100, and 130?μg/mL. Endothelial cell apoptosis showed a dose-dependent increase in response to KDCE and CGA. CGA derivatives from KDCE could have potential as anticancer agents against tumor angiogenesis.

키워드

angiogenesis; chlorogenic acid; endothelial cell apoptosis; kudingcha

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