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Metabolomics Approach in the Study of the Well-Defined Polyherbal Preparation Zyflamend

Journal of Medicinal Food 2018년 21권 3호 p.306 ~ 316
Tague Eric D., Bourdon Allen K., MacDonald Amber, Lookadoo Maggie S., Kim Edward D., White Wesley M., Terry Paul D., Campagna Shawn R., Voy Brynn H., Whelan Jay,
소속 상세정보
 ( Tague Eric D. ) - University of Tennessee Knoxville Department of Chemistry
 ( Bourdon Allen K. ) - University of Tennessee Knoxville Department of Chemistry
 ( MacDonald Amber ) - University of Tennessee Knoxville Department of Nutrition
 ( Lookadoo Maggie S. ) - University of Tennessee Knoxville Department of Chemistry
 ( Kim Edward D. ) - University of Tennessee Medical Center Department of Surgery
 ( White Wesley M. ) - University of Tennessee Medical Center Department of Surgery
 ( Terry Paul D. ) - University of Tennessee Medical Center Department of Medicine
 ( Campagna Shawn R. ) - University of Tennessee Knoxville Department of Chemistry
 ( Voy Brynn H. ) - University of Tennessee Knoxville Department of Animal Science
 ( Whelan Jay ) - University of Tennessee Knoxville Department of Nutrition

Abstract


Zyflamend is a highly controlled blend of 10 herbal extracts that synergistically impact multiple cell signaling pathways with anticancer and anti-inflammatory properties. More recently, its effects were shown to also modify cellular energetics, for example, activation of fatty acid oxidation and inhibition of lipogenesis. However, its general metabolic effects in vivo have yet to be explored. The objective of this study was to characterize the tissue specific metabolomes in response to supplementation of Zyflamend in mice, with a comparison of equivalent metabolomics data generated in plasma from humans supplemented with Zyflamend. Because Zyflamend has been shown to activate AMPK, the “energy sensor” of the cell, in vitro, the effects of Zyflamend on adiposity were also tested in the murine model. C57BL/6 mice were fed diets that mimicked the macro- and micronutrient composition of the U.S. diet with and without Zyflamend supplementation at human equivalent doses. Untargeted metabolomics was performed in liver, skeletal muscle, adipose, and plasma from mice consuming Zyflamend and in plasma from humans supplemented with Zyflamend at an equivalent dose. Adiposity in mice was significantly reduced in the Zyflamend-treated animals (compared with controls) without affecting body weight or weight gain. Based on KEGG pathway enrichment, purine and pyrimidine metabolism (potential regulators of AMPK) were particularly responsive to Zyflamend across all tissues, but only in mice. Consistent with the metabolomics data, Zyflamend activated AMPK and inhibited acetyl CoA-carboxylase in adipose tissue, key regulators of lipogenesis. Zyflamend reduces adipose tissue in mice through a mechanism that likely involves the activation of AMPK.

키워드

adipose; AMPK; metabolomics; Zyflamend

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