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The Red Alga Gracilariopsis chorda and Its Active Constituent Arachidonic Acid Promote Spine Dynamics via Dendritic Filopodia and Potentiate Functional Synaptic Plasticity in Hippocampal Neurons

Journal of Medicinal Food 2018년 21권 5호 p.481 ~ 488
Mohibbullah Md., 최재숙, Bhuiyan Mohammad Maqueshudul Haque, Haque Md. Nazmul, Rahman Md. Khalilur, 문일수, 홍용기,
소속 상세정보
 ( Mohibbullah Md. ) - Pukyong National University Department of Biotechnology
최재숙 ( Choi Jae-Suk ) - Silla University Division of Bioindustry
 ( Bhuiyan Mohammad Maqueshudul Haque ) - Dongguk University College of Medicine Department of Anatomy
 ( Haque Md. Nazmul ) - Dongguk University College of Medicine Department of Anatomy
 ( Rahman Md. Khalilur ) - University of Sydney School of Medicine Department of Pharmacology
문일수 ( Moon Il-Soo ) - Dongguk University College of Medicine Department of Anatomy
홍용기 ( Hong Yong-Ki ) - Pukyong National University Department of Biotechnology

Abstract


Exogenous neurotrophins can induce neuronal differentiation, outgrowth, survival, and synaptic function in the central nervous system. In primary cultures of rat hippocampal neurons, an ethanol extract of the red alga Gracilariopsis chorda (GCE) and its active compound arachidonic acid (AA) significantly increased the densities of dendritic filopodia and spines, promoted the expression of presynaptic vesicle protein 2 (SV2) and postsynaptic density protein 95 (PSD-95), induced robust synaptogenesis, and increased the expression of cell division control protein 42 (CDC42) and actin-related protein 2 (ARP2), which are important for actin organization in dendritic protrusions, and facilitated presynaptic plasticity by increasing the size of the synaptic vesicle pool at presynaptic nerve terminals. In addition, oral administration of GCE and AA for 10 days, at concentrations of 1?mg/g and 2.2?μg/g body weight, respectively, significantly protected against scopolamine-induced memory impairment in mice by increasing the latency time in the passive avoidance test. These results provide strong scientific evidence that these natural products can be used as neurotrophic substances and/or dietary supplements for the prevention and treatment of memory-related neurological disorders via the reconstruction of axo-dendrites and its synapses.

키워드

arachidonic acid; Gracilariopsis chorda; hippocampal neurons; passive avoidance; synaptogenesis

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