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Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model

Journal of Medicinal Food 2018년 21권 7호 p.716 ~ 725
김정아, 김성학, 김인성, 유다윤, 김성찬, 이승호, 이상석, 윤철휘, 최인순, 조광근,
소속 상세정보
김정아 ( Kim Jeong-A ) - Gyeongnam National University of Science & Technology Department of Animal Resources Technology
김성학 ( Kim Sung-Hak ) - Chonnam National University Department of Animal Science
김인성 ( Kim In-Sung ) - Gyeongnam National University of Science & Technology Department of Animal Resources Technology
유다윤 ( Yu Da-Yoon ) - Gyeongnam National University of Science & Technology Department of Animal Resources Technology
김성찬 ( Kim Sung-Chan ) - Hallym University College of Medicine Department of Biochemistry
이승호 ( Lee Seung-Ho ) - Incheon National University Department of Nano-Bioengineering
이상석 ( Lee Sang-Suk ) - Sunchon National University Department of Animal Science and Technology
윤철휘 ( Yun Cheol-Heui ) - Seoul National University Department of Agricultural Biotechnology
최인순 ( Choi In-Soon ) - Silla University Department of Life Sciences
조광근 ( Cho Kwang-Keun ) - Gyeongnam National University Department of Animal Resources Technology

Abstract


Atopic dermatitis is a chronic and recurrent inflammatory skin disease. Recently, probiotics have been shown to suppress allergic symptoms through immunomodulatory responses. In the present study, combinatorial effects on allergic symptoms were identified in BALB/c mice fed with a mixture of four species of probiotics, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum, and sodium butyrate. Following sensitization with whey protein, the mice were challenged and divided into two groups: (1) mice administered with phosphate-buffered saline as a control and (2) mice administered with the probiotic mixture and sodium butyrate. Allergic symptoms were assessed by measuring ear thicknesses, serum histamine and IL-10 concentrations, and the quantities of leaked Evans blue. T cell differentiation was determined by analyzing the T cells groups in the mesenteric lymph nodes (MLNs) and spleen. To examine changes in the total gut microbiota, total fecal microflora was isolated, species identification was performed by DNA sequencing using Illumina MiSeq, and changes in intestinal beneficial bacteria were analyzed using quantitative polymerase chain reaction. Treatment with the probiotic mixture and sodium butyrate reduced ear thicknesses, the quantity of leaked Evans blue, and serum histamine values, while increasing serum IL-10 values. In the mouse model, the probiotic mixture and sodium butyrate increased Th1 and Treg cell differentiation in MLN and spleen tissues; the ratio of Firmicutes/Bacteroidetes, which is associated with reduction in allergic reactions; and microorganisms that lead to cell differentiation into Treg. These results suggest that the probiotic mixture and sodium butyrate can prevent and alleviate allergic symptoms.

키워드

Galectin9; gut microbiota; Th1 cells/Th2 cells; Treg cells

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