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Notoginseng Radix and Rehmanniae Radix Preparata Extract Combination (YH23537) Reduces Pain and Cartilage Degeneration in Rats with Monosodium Iodoacetate-Induced Osteoarthritis

Journal of Medicinal Food 2018년 21권 8호 p.745 ~ 754
전주연, 나현식, 신장우, 정경아, 서현범, 류재윤, 최정원, 문수진, 박현제, 오세웅, 조미라, 민준기,
소속 상세정보
전주연 ( Jhun Joo-Yeon ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
나현식 ( Na Hyun-Sik ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
신장우 ( Shin Jang-Woo ) - Yuhan R&D Institute
정경아 ( Jung Kyung-Ah ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
서현범 ( Seo Hyeon-Beom ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
류재윤 ( Ryu Jae-Yoon ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
최정원 ( Choi Jeong-Won ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
문수진 ( Moon Su-Jin ) - Catholic University College of Medicine Bucheon St. Mary’s Hospital Department of Internal Medicine
박현제 ( Park Hyun-Je ) - Yuhan R&D Institute
오세웅 ( Oh Se-Woong ) - Yuhan R&D Institute
조미라 ( Cho Mi-La ) - Catholic University Catholic Institute of Medical Sciences Rheumatism Research Center
민준기 ( Min Jun-Ki ) - Catholic University College of Medicine Bucheon St. Mary’s Hospital Department of Internal Medicine

Abstract


Notoginseng Radix and Rehmanniae Radix Preparata have been widely used traditionally for treating inflammatory diseases. This research studies the therapeutic effects of YH23537, the extracts of Notoginseng Radix and Rehmanniae Radix Preparata, on pain and cartilage degeneration in an experimental osteoarthritis (OA) model. Male Wistar rats were inoculated intra-articularly with 3?mg of monosodium iodoacetate (MIA) in the right intra-articular. Four days later, the animals were administrated orally with YH23537 daily for 24 days. Tactile allodynia and weight bearing were measured. Macroscopic and microscopic observations for articular cartilage were performed at the end of the experiment. Protein expression in the joint was determined by immunohistochemistry. The effects of YH23537 on mRNA levels in chondrocytes stimulated with interleukin (IL)-1β were analyzed using random polymerase chain reaction. OA induction was confirmed by significant decrease of paw withdrawal latency, paw withdrawal threshold, and weight bearing compared with the normal group at 3 days after MIA injection. The YH23537-treated groups displayed significant increases in pain thresholds and weight bearing throughout the observation period. The damage to articular cartilage was significantly lessened visually and histopathologically by YH23537 treatment. YH23537 suppressed the expression of metalloproteinase-3, nitrotyrosine, IL-1β and IL-6 increased in OA joints. YH23537 upregulated tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-3 in IL-1β-stimulated human OA chondrocytes. The protein levels of the NF-κBp65 and HIF-2α in the joint tissues were reduced by YH23537. YH23537 exerted antinociceptive effects and cartilage protective effects in experimental OA rats by suppressing oxidative injury, inflammatory mediators, and inducing anabolic factors. We suggest that YH23537 may have efficacy for treating OA in humans.

키워드

inflammatory cytokine; monosodium iodoacetate; osteoarthritis; oxidative stress; YH23537

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