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Aqueous Extract of Humulus japonicus Attenuates Hyperlipidemia and Fatty Liver in Obese Mice

Journal of Medicinal Food 2018년 21권 10호 p.999 ~ 1008
정윤희, 방준석, 강창묵, 고지원, 이호성, 홍석명, 김동석, 박언섭, 정태우, 신용규, 이종혁, 정지훈,
소속 상세정보
정윤희 ( Chung Yoon-Hee ) - Chung-Ang University College of Medicine Department of Anatomy
방준석 ( Bang Joon-Seok ) - Sookmyung Women’s University College of Pharmacy
강창묵 ( Kang Chang-Muk ) - Chung-Ang University College of Medicine Department of Pharmacology
고지원 ( Goh Ji-Won ) - Chung-Ang University College of Medicine Department of Pharmacology
이호성 ( Lee Ho-Sung ) - Chung-Ang University College of Medicine Department of Pharmacology
홍석명 ( Hong Seok-Myeong ) - Chung-Ang University College of Medicine Department of Pharmacology
김동석 ( Kim Dong-Seok ) - Chung-Ang University College of Medicine Department of Biochemistry
박언섭 ( Park Eon-Sub ) - Chung-Ang University College of Medicine Department of Pathology
정태우 ( Jung Tae-Woo ) - Seoul National University Bundang Hospital Research Administration Team
신용규 ( Shin Yong-Kyoo ) - Chung-Ang University College of Medicine Department of Pharmacology
이종혁 ( Lee Jong-Hyuk ) - Hoseo University College of Life and Health Science Department of Pharmaceutical Engineering
정지훈 ( Jeong Ji-Hoon ) - Chung-Ang University College of Medicine Department of Pharmacology

Abstract


In this study, the effects of Humulus japonicus (HJ) aqueous extract on 3T3-L1 preadipocytes and HepG2 cells (in vitro model) as well as on C57BL/6 mice fed on high-fat diet (HFD) (in vivo model) were evaluated. Mice fed on HFD for 12-weeks were taken the HJ water extract (HJW) at various doses, 50, 150, and 250?mg/kg, orally for 8 weeks. We have noticed the accumulation of fat globules in preadipocytes and HepG2 cells using Oil Red O staining. In addition, supplementation with HJW considerably inhibited the weight gain, lipid accumulation, and adipogenesis and decreased the size of subcutaneous adipocytes in 3T3-L1 adipocytes. Furthermore, treatment with HJW improved hyperlipidemia via decreasing the levels of serum triglyceride (TG) and low-density lipoproteins as well as the atherogenic index. Supplementation with HJW could attenuate HFD-induced lipid accumulation, increase the mRNA expressions of fatty acid synthase (FAS) and stearoyl-CoA desaturase (SCD1), and would elevate the levels of serum aspartate aminotransferase and alanine aminotransferase in mice liver. The levels of TG and FAS mRNA in HepG2 cells treated with palmitate were reduced in a dose-dependent manner. In sum, HJW could alleviate the HFD-induced obesity and decrease the dyslipidemia profiles; the keys that could contribute to cardiovascular and nonalcoholic liver diseases.

키워드

dyslipidemia; high-fat diet; Humulus japonicus; nonalcoholic fatty liver disease; obesity

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