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Antiproliferative Activity of Extracts of Campomanesia adamantium (Cambess.) O. Berg and Isolated Compound Dimethylchalcone Against B16-F10 Murine Melanoma

Journal of Medicinal Food 2018년 21권 10호 p.1024 ~ 1034
e Silva Magalli C. B. Lima, Bogo Danielle, Alexandrino Caroline A. F., Perdomo Renata T., Figueiredo Patricia de O., do Prado Pamela R., Garcez Fernanda R., Kadri Monica C. T., Ximenes Thalita V. N., Guimaraes Rita de Cassia A., Sarmento Ulana C., Macedo Maria Ligia R.,
소속 상세정보
 ( e Silva Magalli C. B. Lima ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Bogo Danielle ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Alexandrino Caroline A. F. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Perdomo Renata T. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Figueiredo Patricia de O. ) - Federal University of Mato Grosso do Sul Institute of Chemistry
 ( do Prado Pamela R. ) - Federal University of Mato Grosso do Sul Institute of Chemistry
 ( Garcez Fernanda R. ) - Federal University of Mato Grosso do Sul Institute of Chemistry
 ( Kadri Monica C. T. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Ximenes Thalita V. N. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Guimaraes Rita de Cassia A. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Sarmento Ulana C. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition
 ( Macedo Maria Ligia R. ) - Federal University of Mato Grosso do Sul School of Pharmaceutical Sciences, Food and Nutrition

Abstract


Campomanesia adamantium, a native species of the Brazilian Cerrado, is characterized as a natural source of phenolic compounds and has known potential anticancer activities. This study aimed to evaluate the chemical profile of dichloromethane extracts of pulp (DEGPU) and peel (DEGPE) from the fruits of C. adamantium and to identify compounds with antiproliferative effects in vitro against melanoma cells by sulforhodamine B (SRB) assay, apoptosis induction assay, caspase-3 activation assay, nitric oxide (NO) release in coculture of B16-F10 cells and murine peritoneal macrophages. The chemical profiles of DEGPU and DEGPE were analyzed by high performance liquid chromatography coupled to diode array detector and mass spectrometer using the electrospray ionization interface (HPLC-DAD-ESI-MS/MS). Thirteen compounds were identified in both extracts and the chromatographic study of the most active extract in SRB assay DEGPU (GI50 of 16.17?μg/mL) resulted in the isolation of seven compounds. The isolated compound dimethylchalcone (DMC) had the highest antiproliferative activity against B16-F10 with a GI50 of 7.11?μg/mL. DEGPU extract activated caspase-3 in 29% of cells at 25?μg/mL and caused a 50% decrease in NO release in coculture. DEGPU can be characterized as a source of bioactive compounds such as DMC, as seen from its antiproliferative effect in vitro by inducing B16-F10 cells to undergo apoptosis, essential feature in the search for new anticancer drugs.

키워드

apoptosis; caspase-3; chemical profile; dimethylchalcone; guavira; melanoma; nitric oxide

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