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Synergic Laxative Effects of an Herbal Mixture of Liriope platyphylla, Glycyrrhiza uralensis, and Cinnamomum cassia in Loperamide-Induced Constipation of Sprague Dawley Rats

Journal of Medicinal Food 2019년 22권 3호 p.294 ~ 304
김지은, 윤우빈, 이미림, 최준영, 박진주, 김혜령, 송보람, 홍진태, Song Hyun-Keun, 황대연,
소속 상세정보
김지은 ( Kim Ji-Eun ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
윤우빈 ( Yun Woo-Bin ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
이미림 ( Lee Mi-Lim ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
최준영 ( Choi Jun-Young ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
박진주 ( Park Jin-Ju ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
김혜령 ( Kim Hye-Ryeong ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
송보람 ( Song Bo-Ram ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
홍진태 ( Hong Jin-Tae ) - Chungbuk National University College of Pharmacy
 ( Song Hyun-Keun ) - Changwon National University Biomedical Science Institute
황대연 ( Hwang Dae-Youn ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science

Abstract


Constipation is an acute or chronic illness attributed to various causes, ranging from lifestyle habits to side effects of a disease. To improve the laxative effects of some traditional medicines, herbal mixtures of Liriope platyphylla, Glycyrrhiza uralensis, and Cinnamomum cassia (LGC) were evaluated for their mechanism of action and therapeutic effects in loperamide (Lop)-induced constipated Sprague Dawley rats by examining alterations in excretion parameters, histological structure, mucin secretion, and related protein levels. Food intake and water consumption were constant for all animals. We observed that the Lop+LGC-treated group had significantly greater excretion of stool and urine than was observed in the Lop+Vehicle-treated group. Administration of LGC in the constipation model restored the intestinal transit ratio to normal levels, and increased the number of goblet cells, mucosal layer, and muscle thickness. Mucin secretion was greater in the Lop+LGC-treated group than in the Lop+Vehicle-treated group, and the expression of MUC2 and AQP8 genes were also increased. In addition, reverse transcription polymerase chain reaction and Western blot revealed an increase in the muscarinic acetylcholine receptors (mAChRs) in the Lop+LGC-treated group compared to the Lop+Vehicle-treated group. Furthermore, compared with the Lop+Vehicle-treated group, treatment with LGC reduced the phosphorylation of PKC and PI3K, and expression of Gα protein, but increased levels of IP3. Our results suggest that the traditional herbal mixture of LGC induces a potent laxative effect in Lop-induced constipation through mucosal tissue changes and mucin production. We also demonstrated that the laxative effect of LGC is closely related to the expression of mAChR and its downstream signals, suggesting the possibility of developing a constipation-laxative agent using LGC.

키워드

Cinnamomum cassia; constipation; Glycyrrhiza uralensis; laxative effects; Liriope platyphylla

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