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Orostachys japonicus A. Berger (Crassulaceae) Exerts Antidiabetic Activity by Improving Glucose and Lipid Levels in Type 2 Diabetic Mice

Journal of Medicinal Food 2019년 22권 8호 p.797 ~ 809
정현의, 김종원, Yang Da-Ram, 정태원, Zhao Jing, 서정훈, 신동규, 차정단, 한강민, 임채웅, 김범석,
소속 상세정보
정현의 ( Jeong Hyun-Eui ) - Chonbuk National University College of Veterinary Medicine
김종원 ( Kim Jong-Won ) - Chonbuk National University College of Veterinary Medicine
 ( Yang Da-Ram ) - Chonbuk National University College of Veterinary Medicine
정태원 ( Jeong Tae-Won ) - Chonbuk National University College of Veterinary Medicine
 ( Zhao Jing ) - Chonbuk National University College of Veterinary Medicine
서정훈 ( Seo Jeong-Hun ) - GENERAL BIO Co. Ltd. Research and Development Center
신동규 ( Shin Dong-Gue ) - GENERAL BIO Co. Ltd. Research and Development Center
차정단 ( Cha Jeong-Dan ) - GENERAL BIO Co. Ltd. Research and Development Center
한강민 ( Han Kang-Min ) - Dongguk University Ilsan Hospital Department of Pathology
임채웅 ( Lim Chae-Woong ) - Chonbuk National University College of Veterinary Medicine
김범석 ( Kim Bum-Seok ) - Chonbuk National University College of Veterinary Medicine

Abstract


Orostachys japonicus A. Berger and Momordica charantia Linn have been widely used as an alternative medicine. Recently, patients with type 2 diabetes (T2D) have paid increasing attention to medical nutrition therapy due to its safety and cost-effectiveness. Therefore, we have developed a new health functional food that consists of a mixed extract of O. japonicus and M. charantia. The aim of this study is designed to assess the antidiabetic efficacy of O. japonicus and M. charantia extracts (OME, in an 8:2 ratio), especially focusing on the effects of O. japonicus via in vivo and in vitro experiments. Seven-week-old C57BL/Ksj-db/db (db/db; a genetic animal model of T2D) mice were used for inducing diabetes. Mice were administered with various concentrations of OME (OME 0, 100, 200, or 400?mg/kg/day) for 6 weeks. Metabolic parameters, fasting blood glucose and glycosylated hemoglobin levels were measured. Histopathologic analysis and the levels of serum or hepatic biochemicals were assessed to evaluate diabetic liver injury and steatosis. The expression levels of lipogenic and gluconeogenic genes were determined by quantitative real-time polymerase chain reaction. Activation of Akt was assessed by western blot analysis. Administration of OME significantly improved metabolic parameters in db/db mice, and also reduced diabetic liver injury and steatosis were observed by OME administration in db/db mice as confirmed by histopathologic and serum or hepatic biochemical analysis. Consistently, treatment of OME significantly increased Akt activation resulting in decreased expression levels of lipid-accumulation or gluconeogenesis-related genes. Similar results were observed in in vitro experiments using single extract of O. japonicus and using OME. OME has antidiabetic effects with increased insulin sensitivity, and may be a safe alternative therapy for the management of T2D.

키워드

Akt; insulin resistanceliver; O. japonicus; type 2 diabetes

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