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Inhibition of miR31 and miR92a as Oncological Biomarkers in RKO Colon Cancer Cells Treated with Kaempferol-3-O-Glycoside Isolated from Black Bean

Journal of Medicinal Food 2020년 23권 1호 p.50 ~ 55
Gutierrez-Uribe Janet Alejandra, Salinas-Santander Mauricio, Serna-Guerrero Delia, Serna-Saldivar Sergio Roman Othon, Rivas-Estilla Ana Maria, Rios-Ibarra Clara Patricia,
소속 상세정보
 ( Gutierrez-Uribe Janet Alejandra ) - Tecnologico de Monterrey Department of Bioengineering and Science
 ( Salinas-Santander Mauricio ) - Autonomous University of Coahuila
 ( Serna-Guerrero Delia ) - Tecnologico de Monterrey Protein Research and Development Center
 ( Serna-Saldivar Sergio Roman Othon ) - Tecnologico de Monterrey Protein Research and Development Center
 ( Rivas-Estilla Ana Maria ) - Autonomous University of Nuevo Leon School of Medicine Department of Biochemistry and Molecular Medicine
 ( Rios-Ibarra Clara Patricia ) - Tecnologico de Monterrey Department of Bioengineering

Abstract


MicroRNAs (miRNAs) are small molecules of 19?23 nucleotides of RNA that act as regulators of the expression of proteins in eukaryotic cells. Currently, the participation of miRNAs in the development of different types of cancer has been observed. To evaluate the inhibitory effect of kaempferol-3-O-glycoside on the expression of oncological biomarkers, miR31 and miR92a in a colon cancer cell line (RKO) were analyzed. Cells were cultured and treated with 1?mM kaempferol-3-O-glycoside isolated from black bean. Expression levels of miR31 and miR92a were evaluated by real-time PCR using TaqMan probes; in addition, two oncogenes (KRAS and c-MYC) and two tumor suppressors (AMP-activated protein kinase [AMPK] and adenomatous tumors of polyposis coli [APC]) were quantified to validate the biological effects; normalization of expression levels were carried out by 2?ΔΔCt. Results were analyzed by one-way ANOVA. The expression levels of miR31, miR92a, KRAS oncogene, and the c-MYC transcription factor were subexpressed upon 72?h post-treatment with kaempferol-3-O-glycoside compared with the control without treatment (P < .05); in contrast, the tumor suppressor genes AMPK (∼4.85, P?=?.005) and APC (∼2.71, P?=?.066) tumor suppressors genes were overexpressed. Our results showed the inhibitory effect of isolated black bean flavonoid kaempferol-3-O-glycoside on cancer biomarkers: miR31 and miR92a; based on our results, this flavonoid may have interesting nutritional, therapeutic, and/or prophylactic applications to combat colon cancer.

키워드

black bean; colon cancer; kaempferol; microRNAs; miR31; miR92a

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