잠시만 기다려 주세요. 로딩중입니다.

Structural Evidence for Antihypertensive Effect of an Antioxidant Peptide Purified from the Edible Marine Animal Styela clava

Journal of Medicinal Food 2020년 23권 2호 p.132 ~ 138
강나래, 고석천, 김현수, 양혜원, 안긴내, 이승철, Lee Tae-Gee, 이정석, 전유진,
소속 상세정보
강나래 ( Kang Na-Lae ) - Jeju National University Department of Marine Life Science
고석천 ( Ko Seok-Chun ) - National Marine Biodiversity Institute of Korea Team of Marine Bio-resources
김현수 ( Kim Hyun-Soo ) - Jeju National University Department of Marine Life Science
양혜원 ( Yang Hye-Won ) - Jeju National University Department of Marine Life Science
안긴내 ( Ahn Gin-Nae ) - Chonnam National University Department of Marine Bio-Food Sciences
이승철 ( Lee Seung-Cheol ) - Kyungnam University Department Food Science and Biotechnology
 ( Lee Tae-Gee ) - Jeonnam State University Department of Hotel Cuisine and Baking
이정석 ( Lee Jung-Suck ) - Gyeongsang National University Research Center for Industrial Development of Seafood
전유진 ( Jeon You-Jin ) - Jeju National University Department of Marine Life Science

Abstract


This study investigated the antihypertensive effects of an antioxidant peptide, Leu-Trp-His-Thr-His (LWHTH), purified from Styela clava peptic hydrolysate, to assess the bioactivity of the peptide and verify the value of S. clava as a health-promoting food. Also, the study presented structural evidence for the effects of LWHTH. The inhibitory effect of LWHTH on angiotensin I-converting enzyme (ACE) was assessed using enzyme reaction methods and the simulation methods in computational space. LWHTH inhibited ACE with an IC50 value of 16.42?±?0.45?μM. The LWHTH structure was stable, and its ACE inhibitory effect was retained under simulated gastrointestinal conditions. In silico simulations revealed that LWHTH binds the active site of ACE, with residues LW making the ACE?LWHTH complex stable and residues HTH making the complex strong. Furthermore, LWHTH significantly reduced blood pressure in spontaneously hypertensive rats. These results demonstrate that LWHTH has the potential to be a healthy functional food with antihypertensive effects. Therefore, S. clava consumption may be beneficial for human health.

키워드

angiotensin I-converting enzyme; antioxidant peptide; blood pressure; molecular docking; Styela clava

원문 및 링크아웃 정보

등재저널 정보