잠시만 기다려 주세요. 로딩중입니다.

Therapeutic Potential of Bauhinia forficata Link in Dental Biofilm Treatment

Journal of Medicinal Food 2020년 23권 9호 p.998 ~ 1005
Ferreira-Filho Julio Cesar C., Marre Andressa Temperini de Oliveira, Almeida Juliana Soares de Sa, Lobo Leandro de Araujo, Farah Adriana, Romanos Maria Teresa Villela, Maia Lucianne Cople, Valenca Ana Maria Gondim, Fonseca-Goncalves Andrea,
소속 상세정보
 ( Ferreira-Filho Julio Cesar C. ) - Federal University of Rio de Janeiro School of Dentistry Department of Pediatric Dentistry and Orthodontics
 ( Marre Andressa Temperini de Oliveira ) - UFRJ Institute of Microbiology Department of Medical Microbiology
 ( Almeida Juliana Soares de Sa ) - UFRJ Institute of Microbiology Department of Medical Microbiology
 ( Lobo Leandro de Araujo ) - UFRJ Institute of Microbiology Department of Medical Microbiology
 ( Farah Adriana ) - UFRJ Institute of Nutrition
 ( Romanos Maria Teresa Villela ) - UFRJ Institute of Microbiology Department of Virology
 ( Maia Lucianne Cople ) - Federal University of Rio de Janeiro School of Dentistry Department of Pediatric Dentistry and Orthodontics
 ( Valenca Ana Maria Gondim ) - Federal University of Paraiba School of Dentistry Department of Clinic and Social Dentistry
 ( Fonseca-Goncalves Andrea ) - Federal University of Rio de Janeiro School of Dentistry Department of Pediatric Dentistry and Orthodontics

Abstract


The oral cytotoxicity, antimicrobial and anti-demineralizing effects of a tincture from Bauhinia forficata Link tincture (BFLT) were evaluated in vitro and ex vivo. Susceptibility tests (minimum inhibitory and microbicidal concentrations-MIC and time-kill assay-MMC) were performed against planktonic oral microorganisms. The contents of phenolic compounds were investigated. Cytotoxic potential was evaluated on oral fibroblasts after 1?5?min exposure to BFLT. Blocks of sound bovine enamel (N?=?60) were inoculated with a saliva pool and sustained in a multiple plaque growth system for 48?h to form a biofilm. Biofilm blocks were randomly divided into groups-G (n?=?10): G1-Baseline (48?h maturation biofilm), G2-BFLT 23.2?mg/mL, G3-Ethanol 81.20?g/mL, G4-Chlorhexidine 0.12%, G5-Growth control, and G6-Blank control. Treatments (50?μL/1?min) were performed once a day for a week. Streptococcus spp. (S) and total microorganism (TM) counts were expressed as Log10 CFU/mL. Biofilm height was evaluated by confocal microscopy analyses (CMA). Final surface hardness was assessed and percentage of microhardness loss (% MHL) was calculated. Results were significant when P?

키워드

Bauhinia forficata Link; dental plaque; microbiology; microhardness; phytotherapy; tooth

원문 및 링크아웃 정보

등재저널 정보