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Protective Effect of Glycomacropeptide on the Atopic Dermatitis-Like Dysfunctional Skin Barrier in Rats

Journal of Medicinal Food 2020년 23권 11호 p.1216 ~ 1224
Jimenez Mariela, Munoz Fabiola C., Cervantes-Garcia Daniel, Cervantes Maritza M., Hernandez-Mercado Alicia, Barron-Garcia Berenice, Hernandez-Duque Jose L. Moreno, Rodriguez-Carlos Adrian, Rivas-Santiago Bruno, Salinas Eva,
소속 상세정보
 ( Jimenez Mariela ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Munoz Fabiola C. ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Cervantes-Garcia Daniel ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Cervantes Maritza M. ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Hernandez-Mercado Alicia ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Barron-Garcia Berenice ) - Autonomous University of Aguascalientes Department of Microbiology
 ( Hernandez-Duque Jose L. Moreno ) - Autonomous University of Aguascalientes Department of Chemistry
 ( Rodriguez-Carlos Adrian ) - Mexican Institute of Social Security Medical Research Unit from Zacatecas
 ( Rivas-Santiago Bruno ) - Mexican Institute of Social Security Medical Research Unit from Zacatecas
 ( Salinas Eva ) - Autonomous University of Aguascalientes Department of Microbiology

Abstract


The maintenance of a healthy skin barrier is crucial to prevent and treat atopic dermatitis (AD) lesions and avoid infections. Glycomacropeptide (GMP) is a bioactive peptide that has demonstrated promising results as an anti-inflammatory and antipruritic therapy for experimental AD. This study aimed to analyze the effect of GMP on impaired cutaneous barrier-related signs in a rat model of AD lesions. AD-like dermatitis was induced on the skin by repeated topical applications of 2,4-dinitrochlorobenzene, and animals were orally administered GMP before or after AD induction. The expression of skin structural proteins and antimicrobial peptides (AMPs) was evaluated by immunoblot or immunohistochemistry, epidermal thickening was evaluated by histochemistry, the level of IFN-γ and changes in the microbiota were evaluated by quantitative polymerase chain reaction, and the quantity of fecal short-chain fatty acids (SCFAs) was evaluated by gas chromatography. GMP administration significantly increased filaggrin, β-defensin 2, and cathelicidin-related AMP expression in AD-like lesions. Involucrin expression was not modified. In GMP-treated animals, epidermal thickening and IFN-γ expression were strongly reduced in damaged skin. GMP treatment impacted the skin microbiota and prevented Staphylococcus aureus colonization, which is associated with AD. In addition, high levels of Bifidobacterium were detected in the feces of GMP-treated animals, and the acetic acid and butyric acid contents increased in animals prophylactically administered GMP. These results suggest that GMP markedly prevents or reverses skin barrier damage in rat AD-like lesions through a bifidogenic effect that induces fecal SCFA production with prolonged treatment. Our findings provide evidence that GMP may represent an optimum strategy for the therapy of the dysfunctional cutaneous barrier in AD.

키워드

antimicrobial peptides; Bifidobacterium; epidermal thickness; short-chain fatty acids; Staphylococcus aureus; stratum corneum

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