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The Impact of Genistein Supplementation on Tendon Functional Properties and Gene Expression in Estrogen-Deficient Rats

Journal of Medicinal Food 2020년 23권 12호 p.1266 ~ 1274
Carroll Chad C., Patel Shivam H., Simmons Jessica, Gordon Ben D. H., Olson Jay F., Chemelewski Kali, Saw Shannon, Hale Taben M., Howden Reuben, Sabbaghi Arman,
소속 상세정보
 ( Carroll Chad C. ) - Purdue University Department of Health and Kinesiology
 ( Patel Shivam H. ) - Purdue University Department of Health and Kinesiology
 ( Simmons Jessica ) - Purdue University Department of Health and Kinesiology
 ( Gordon Ben D. H. ) - University of North Carolina at Charlotte Department of Kinesiology
 ( Olson Jay F. ) - Midwestern University Department of Physiology
 ( Chemelewski Kali ) - Purdue University Department of Health and Kinesiology
 ( Saw Shannon ) - Purdue University Department of Health and Kinesiology
 ( Hale Taben M. ) - University of Arizona College of Medicine Department of Basic Medical Sciences
 ( Howden Reuben ) - University of North Carolina at Charlotte Department of Kinesiology
 ( Sabbaghi Arman ) - Purdue University Department of Statistics

Abstract


Tendinopathy risk increases with menopause. The phytoestrogen genistein prevents collagen loss during estrogen deficiency (ovariectomy [OVX]). The influence of genistein on tendon function and extracellular matrix (ECM) regulation is not well known. We determined the impact of genistein on tendon function and the expression of several genes important for the regulation of tendon ECM. Eight-week-old rats (n?=?42) were divided into three groups: intact, OVX, or OVX-genistein (6?mg/kg/day) for 6 weeks. Tail fascicles were assessed with a Deben tensile stage. Achilles tendon mRNA expression was determined with digital droplet polymerase chain reaction. Compared to intact, fascicle stress tended to be lower in untreated OVX rats (P?=?.022). Furthermore, fascicle modulus and energy density were greater in genistein-treated rats (P??.05). Compared to intact, Tnmd and Esr1 expression were greater and Pcna and Timp1 expression were lower in OVX rats (P??.05). Several β-catenin/Wnt signaling-related molecules were not altered by OVX or genistein (P?>?.05). Our findings demonstrate that genistein improves tendon function in estrogen-deficient rats. The effect of genistein in vivo was predominately on genes related to cell proliferation rather than collagen remodeling.

키워드

collagen; estrogen deficiency; extracellular matrix; genistein; rat; tendon

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