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Xinhuang Tablets Improve Intestinal Barrier Function via Regulating Epithelial Tight Junctions in Dextran Sulfate Sodium-Induced Ulcerative Colitis Mice

Journal of Medicinal Food 2021년 24권 1호 p.33 ~ 39
Wu Meizhu, Shen Aling, Chen Youqin, Liu Liya, Li Li, Sankararaman Senthilkumar, Chen Hongwei, Guan Bin, Zhan Zhixue, Nan Shuhua, Sferra Thomas J., Peng Jun,
소속 상세정보
 ( Wu Meizhu ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine
 ( Shen Aling ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine
 ( Chen Youqin ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine
 ( Liu Liya ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine
 ( Li Li ) - Fujian Provincial Hospital Department of Health Management
 ( Sankararaman Senthilkumar ) - Case Western Reserve University School of Medicine Rainbow Babies and Children’s Hospital Department of Pediatrics
 ( Chen Hongwei ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine
 ( Guan Bin ) - Xiamen Traditional Chinese Medicine Co. Ltd.
 ( Zhan Zhixue ) - Xiamen Traditional Chinese Medicine Co. Ltd.
 ( Nan Shuhua ) - Xiamen Traditional Chinese Medicine Co. Ltd.
 ( Sferra Thomas J. ) - Case Western Reserve University School of Medicine Rainbow Babies and Children’s Hospital Department of Pediatrics
 ( Peng Jun ) - Fujian University of Traditional Chinese Medicine Academy of Integrative Medicine

Abstract


Intestinal mucosal barrier dysfunction is involved in the pathogenesis of inflammatory bowel disease, including ulcerative colitis (UC). Xinhuang tablets (XHTs) have been prescribed for several kinds of inflammatory diseases, including UC, whereas its possible underlying molecular mechanisms had never been explored. Mouse model of UC was constructed by DSS treatment and followed by XHT treatment. Disease activity index, histopathological of colonic tissue, tumor necrosis factor-alpha (TNF-α), and serum amyloid A (SAA) levels in serum were further assessed. The underlying mechanism was further explored by determination of the expression of epithelial tight junction-related protein. XHT administration ameliorated dextran sulfate sodium (DSS)-induced clinical symptoms, colonic histological injury, and decreased the circulating levels of TNF-α and SAA. Moreover, XHT treatment significantly increased the protein levels of zona occludens (ZO)-1, whereas decreased the levels of phosphorylation of Elk-1. In conclusion, this study confirmed the therapeutic effects of XHT treatment on UC in a DSS-induced mouse model, and indicated that by increasing expression of epithelial tight junctions and decreasing phosphorylation of Elk-1 might be one of the underlying mechanisms of XHT treatment on UC.

키워드

inflammatory bowel disease; intestinal barrier; tight junction; traditional Chinese medicine; Xinhuang tablets

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