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Cenostigma macrophyllum Tul. var. acuminata Teles Freire Fraction Leaves Stimulate Gastric Healing in Rats and Human Cell Cultures

Journal of Medicinal Food 2021년 24권 3호 p.248 ~ 257
Viana Ana Flavia Seraine Custodio, Fernandes Helio B., Chaves Mariana H., Viana Daniel A., Santos Verlane G., Silva Ariadne C. A., Lopes Miriam T. P., Oliveira Rita de Cassia M.,
소속 상세정보
 ( Viana Ana Flavia Seraine Custodio ) - Federal University of Piaui Medicinal Plants Research Center
 ( Fernandes Helio B. ) - Federal University of Piaui Medicinal Plants Research Center
 ( Chaves Mariana H. ) - Federal University of Piaui Department of Chemistry
 ( Viana Daniel A. ) - State University of Ceara Pathology Laboratory and Forensic Medicine-Favet
 ( Santos Verlane G. ) - Federal University of Minas Gerais Department of Pharmacology
 ( Silva Ariadne C. A. ) - Federal University of Minas Gerais Department of Pharmacology
 ( Lopes Miriam T. P. ) - Federal University of Minas Gerais Department of Pharmacology
 ( Oliveira Rita de Cassia M. ) - Federal University of Piaui Medicinal Plants Research Center

Abstract


Cenostigma macrophyllum Tul. var. acuminata Teles Freire (Leguminosae?Caesalpinioideae) is a medicinal plant traditionally used for treatment of gastric ulcer. This study evaluated the ulcer-healing activity of the hydroalcoholic fraction of C. macrophyllum Tul. var. acuminata Teles Freire leaves (Cm-FHA) and the tea of the leaves of C. macrophyllum (Cm-tea), as well as the possible action of Cm-FHA, through in vitro models. Leaves of C. macrophyllum were dried and powdered to obtain the Cm-FHA. Subsequently, the Cm-FHA was characterized phytochemically and biologically. Besides, Cm-tea was prepared. The gastric healing effects of Cm-tea and Cm-FHA were analyzed using the model of acetic acid-induced gastric ulcer in rats. The human gastric adenocarcinoma (AGS) cell line was employed as an in vitro model. Cm-tea promoted a protective effect against gastric ulcers induced by absolute ethanol. Cm-FHA or Cm-tea (100?mg/kg/7 days) exhibited a significant healing effect on ulcers induced by acetic acid. In the histological analysis, gastric mucosa treated with Cm-FHA or Cm-tea advanced restoration of the mucosal epithelium. In vitro, lower concentrations of Cm-FHA stimulated cell proliferation in the BrdU assay and cell migration. Cm-tea and Cm-FHA present a significant gastric healing effect in in vivo and in vitro models.

키워드

gastric ulcer; hydroalcoholic fraction; tea; wound healing

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