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RAW 264.7 cell에서의 미모사 에탄올 추출물의 항산화 및 항염증 효능 연구

Antioxidant and Anti-Inflammatory Effects of Mimosa Pudica Ethanol Extract in RAW 264.7 Cell

동물실험대체법학회지 2020년 14권 1호 p.49 ~ 57
전민영, 김배환,
소속 상세정보
전민영 ( Jeon Min-Yeong ) - Keimyung University College of Natural Sciences Department of Public Health
김배환 ( Kim Bae-Hwan ) - Keimyung University College of Natural Sciences Department of Public Health

Abstract


The purpose of this study is to identify the possibility of Mimosa pudica ethanol extracts (MEE) as a cosmeceutical material with antioxidant and anti-inflammatory effects. At first, the antioxidant effect of the MEE was carried out through Total polyphenol, 2,2diphenyl-1- picrylhydrazyl (DPPH) electro-donating activity, 2,2’-azino-bis diammonium salt (ABTS+) radial scavenging activity. The total polyphenol content of the MEE was 3.514 mg/g. DPPH electro-donating activity of MEE were increased in a dose-dependent manner (P<0.05), and was about 88.1% at 1 mg/mL concentration. Also, ABTS+ radical scavenging activity of MEE were remarkably increased in a dose-dependent manner (P<0.05), and was about 99.7% at 1 mg/mL concentration. The MEE showed relatively high antioxidant effects in most experiments. To evaluate the anti-inflammatory effects in RAW 264.7 cells, the maximum permissible concentration was verified first by cell viability assessment using MTT assay. And then, nitric oxide (NO) production inhibition and amounts of inflammatory related proteins and gene expressions were identified after LPS treatment. NO inhibition of MEE was decreased concentration dependently, and was about 84.3% at 1 mg/mL concentration. And TNF-α and IL-6 proteins by western blotting were also decreased significantly by treatment of MEE. As a result of measuring the expression levels of TNF-α and IL-6, the expression inhibition rates were 67.87% and 71.91%, respectively. Based on the results of these experiments, we suggest that MEE could be useful as a cosmeceutical material with anti-inflammatory effects.

키워드

Mimosa pudica; antioxidant, anti-inflammatory; TNF-α; IL-6

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