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Vimentin Deficiency Prevents High-Fat Diet-Induced Obesity and Insulin Resistance in Mice

Diabetes & Metabolism Journal 2021년 45권 1호 p.97 ~ 108
김서연, 김인영, 조원경, 오구택, 박영미,
소속 상세정보
김서연 ( Kim Seo-Yeon ) - Ewha Womans University College of Medicine Department of Molecular Medicine
김인영 ( Kim In-Yeong ) - Ewha Womans University College of Medicine Department of Molecular Medicine
조원경 ( Cho Won-Kyoung ) - Ewha Womans University College of Medicine Department of Molecular Medicine
오구택 ( Oh Goo-Taeg ) - Ewha Womans University Department of Life Sciences
박영미 ( Park Young-Mi ) - Ewha Womans University College of Medicine Department of Molecular Medicine

Abstract


Background: Obesity and type 2 diabetes mellitus are world-wide health problems, and lack of understanding of their linking mechanism is one reason for limited treatment options. We determined if genetic deletion of vimentin, a type 3 intermediate filament, affects obesity and type 2 diabetes mellitus.

Methods: We fed vimentin-null (Vim?/?) mice and wild-type mice a high-fat diet (HFD) for 10 weeks and measured weight change, adiposity, blood lipids, and glucose. We performed intraperitoneal glucose tolerance tests and measured CD36, a major fatty acid translocase, and glucose transporter type 4 (GLUT4) in adipocytes from both groups of mice.

Results: Vim?/? mice fed an HFD showed less weight gain, less adiposity, improved glucose tolerance, and lower serum level of fasting glucose. However, serum triglyceride and non-esterified fatty acid levels were higher in Vim?/? mice than in wild-type mice. Vimentin-null adipocytes showed 41.1% less CD36 on plasma membranes, 27% less uptake of fatty acids, and 50.3% less GLUT4, suggesting defects in intracellular trafficking of these molecules.

Conclusion: We concluded that vimentin deficiency prevents obesity and insulin resistance in mice fed an HFD and suggest vimentin as a central mediator linking obesity and type 2 diabetes mellitus.

키워드

CD36 antigens; Glucose transporter type 4; Insulin resistance; Obesity; Vimentin

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