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Influence of three BALB/c substrain backgrounds on the skin tumor induction efficacy to DMBA and TPA cotreatment

Laboratory Animal Research 2020년 36권 1호 p.30 ~ 30
강미주, 공정은, 김지은, 최현준, 배수지, 최윤주, 이수진, 서민수, 김길수, 정영석, 조준용, 임용, 황대연,
소속 상세정보
강미주 ( Kang Mi-Ju ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
공정은 ( Gong Jeong-Eun ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
김지은 ( Kim Ji-Eun ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
최현준 ( Choi Hyeon-Jun ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
배수지 ( Bae Su-Ji ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
최윤주 ( Choi Yun-Ju ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
이수진 ( Lee Su-Jin ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science
서민수 ( Seo Min-Soo ) - Daegu-Gyeongbuk Medical Innovation Foundation Laboratory Animal Center
김길수 ( Kim Kil-Soo ) - Daegu-Gyeongbuk Medical Innovation Foundation Laboratory Animal Center
정영석 ( Jung Young-Suk ) - Pusan National University College of Pharmacy
조준용 ( Cho Joon-Yong ) - Korea National Sport University Exercise Biochemistry Laboratory
임용 ( Lim Yong ) - Dong-Eui University College of Nursing & Healthcare Science Department of Clinical Laboratory Science
황대연 ( Hwang Dae-Youn ) - Pusan National University College of Natural Resources and Life Science Department of Biomaterials Science

Abstract


Differences in responsiveness of BALB/c substrains have been investigated in various fields, including diabetes induction, corpus callosum deficiency, virus-induced demyelinating disease, aggressive behavior and osteonecrosis. However, induction efficacy of skin tumor remains untried. We therefore investigated the influence of BALB/c substrain backgrounds on the skin tumor induction efficacy in response to DMBA (7,12-Dimethylbenz[a]anthracene) and TPA (12-O-tetradecanoylphorbol-13-acetate) cotreatment. Alterations in the levels of tumor growth related factors, histopathological structure, and the expression to tumor related proteins were measured in three BALB/c substrains (BALB/cKorl, BALB/cA and BALB/cB) after exposure to DMBA (25?μg/kg) and three different doses of TPA (2, 4 and 8?μg/kg). The average number and induction efficacy of tumors in response to DMBA+TPA treatment were significantly greater in the BALB/cKorl substrain than in BALB/cA and BALB/cB. However, cotreatment with DMBA+TPA induced similar responses for body and organ weights of all three substrains. Few differences were detected in the serum analyzing factors, while similar responsiveness was observed for blood analyzing factors after DMBA+TPA treatment. Furthermore, the three BALB/c substrains exhibited similar patterns in their histopathological structure in DMBA+TPA-induced tumors. The expression levels of apoptotic proteins and tumor related proteins were constantly maintained in all three BALB/c substrains treated with DMBA+TPA. In addition, the responsiveness to cisplatin treatment was overall very similar in the three BALB/c substrains with DMBA+TPA-induced tumors. Taken together, these results indicate that genetic background of the three BALB/c substrains does not have a major effect on the DMBA+TPA-induced skin carcinogenesis and therapeutic responsiveness of cisplatin, except induction efficacy.

키워드

BALB/c; BALB/cKorl; Substrains; DMBA+TPA; Cisplatin; Skin tumor

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