잠시만 기다려 주세요. 로딩중입니다.

Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron

Laboratory Animal Research 2020년 36권 1호 p.31 ~ 31
진현정, 이소영, 이대기,
소속 상세정보
진현정 ( Chin Hyun-Jung ) - Ewha Womans University Department of Life Sciences
이소영 ( Lee So-Young ) - Ewha Womans University Department of Life Sciences
이대기 ( Lee Dae-Kee ) - Ewha Womans University Department of Life Sciences

Abstract


Genetically engineered mouse models through gene deletion are useful tools for analyzing gene function. To delete a gene in a certain tissue temporally, tissue-specific and tamoxifen-inducible Cre transgenic mice are generally used. Here, we generated transgenic mouse with cardiac-specific expression of Cre recombinase fused to a mutant estrogen ligand-binding domain (ERT2) on both N-terminal and C-terminal under the regulatory region of human vasoactive intestinal peptide receptor 2 (VIPR2) intron and Hsp68 promoter (VIPR2-ERT2CreERT2). In VIPR2-ERT2CreERT2 transgenic mice, mRNA for Cre gene was highly expressed in the heart. To further reveal heart-specific Cre expression, VIPR2-ERT2CreERT2 mice mated with ROSA26-lacZ reporter mice were examined by X-gal staining. Results of X-gal staining revealed that Cre-dependent recombination occurred only in the heart after treatment with tamoxifen. Taken together, these results demonstrate that VIPR2-ERT2CreERT2 transgenic mouse is a useful model to unveil a specific gene function in the heart.

키워드

VIPR2 intron; ERT2CreERT2; Heart; Tamoxifen-inducible Cre transgenic mouse

원문 및 링크아웃 정보

등재저널 정보

KCI
KoreaMed