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Decrease of protein phosphatase 2A subunit B by glutamate exposure in the cerebral cortex of neonatal rats

Laboratory Animal Research 2020년 36권 1호 p.34 ~ 34
강주빈, 박동주, 손현경, 고필옥,
소속 상세정보
강주빈 ( Kang Ju-Bin ) - Gyeongsang National University College of Veterinary Medicine Department of Anatomy
박동주 ( Park Dong-Ju ) - Gyeongsang National University College of Veterinary Medicine Department of Anatomy
손현경 ( Son Hyun-Kyoung ) - Gyeongsang National University College of Veterinary Medicine Department of Anatomy
고필옥 ( Koh Phil-Ok ) - Gyeongsang National University College of Veterinary Medicine Department of Anatomy

Abstract


Glutamate induces neurotoxicity during brain development, causing nerve damage. Protein phosphatase 2A (PP2A) is a type of serine/threonine phosphatase that regulates various biological functions. Among the PP2A subunit types, subunit B is abundant in brain tissue and plays an essential role in the nervous system. This study investigated changes in PP2A subunit B expression through glutamate exposure in the cerebral cortex of newborn rats. Sprague-Dawley rat pups (7 days after birth) were injected intraperitoneally with vehicle or glutamate (10 mg/kg). After 4 h of drug treatment, the brain tissue was isolated and fixed for morphological study. In addition, the cerebral cortex was collected for RNA and protein works. We observed severe histopathological changes including swollen neuron and atrophied dendrite in the glutamate exposed cerebral cortex. Glutamate exposure leads to a decrease in PP2A subunit B. Reverse-transcription PCR and Western blot analyses confirmed that glutamate induces a decrease of PP2A subunit B in the cerebral cortex of newborn rats. Moreover, immunohistochemical study showed a decrease in PP2A subunit B positive cells. The reduction of PP2A subunit B expression is considered an indicator of neurodegenerative damage. These results suggest that glutamate exposure causes neuronal damage in the cerebral cortex of new born rats through a decrease in PP2A subunit B.

키워드

Cerebral cortex; Glutamate; Neonate; PP2A

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