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대사공학에 의해 개발된 코리네박테리움 글루타미컴에 의한 4-히드록시벤질 알코올 생산

Production of 4-Hydroxybenzyl Alcohol Using Metabolically Engineered Corynebacterium glutamicum

Microbiology and Biotechnology Letters 2020년 48권 4호 p.506 ~ 514
김부연, 정혜빈, 이지영, Ferrer Lenny, Purwanto Henry Syukur, 이진호,
소속 상세정보
김부연 ( Kim Bu-Yeon ) - Kyungsung University School of Food Biotechnology and Nutrition Major in Food Biotechnology
정혜빈 ( Jung Hye-Bin ) - GeneChem Inc.
이지영 ( Lee Ji-Yeong ) - Kyungsung University School of Food Biotechnology and Nutrition Major in Food Biotechnology
 ( Ferrer Lenny ) - Bielefeld University Faculty of Biology
 ( Purwanto Henry Syukur ) - Kyungsung University School of Food Biotechnology and Nutrition Major in Food Biotechnology
이진호 ( Lee Jin-Ho ) - Kyungsung University School of Food Biotechnology and Nutrition Major in Food Biotechnology

Abstract


4-Hydroxybenzyl alcohol (4-HB alcohol) is one of the major active components of Gastrodia elata Blume, with beneficial effects on neurological disorders such as headache, convulsive behavior, and dizziness. Here, we developed a metabolically engineered Corynebacterium glutamicum strain able to produce 4-HB alcohol from 4-hydroxybenzoate (4-HBA). First, the strain APS963 was obtained from the APS809 strain via the insertion of aroK from Methanocaldococcus jannaschii into the NCgl2922-deleted locus. As carboxylic acid reductase from Nocardia iowensis catalyzes the reduction of 4HBA to 4-hydroxybenzaldehyde (4-HB aldehyde), we then introduced a codon-optimized car gene into the genome of APS963, generating the GAS177 strain. Then, we deleted creG coding for a putative short-chain dehydrogenase and inserted ubiCpr encoding a product-resistant chorismate-pyruvate lyase into the pcaHG-deleted locus. The resulting engineered GAS355 strain accumulated 2.3 g/l 4-HB alcohol with 0.32 g/l 4-HBA and 0.3 g/l 4-HB aldehyde as byproducts from 8% glucose after 48 h of culture.

키워드

4-hydroxybenzyl alcohol; Gastrodia elata; Corynebacterium glutamicum; metabolic engineering; carboxylic acid reductase

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